RT Journal Article
T1 Tetrahymena Glutathione peroxidase family: a comparative analysis of these antioxidant enzymes and differential gene expression to metalsand Oxidizing Agents
A1 Cubas-Gaona, Liliana L.
A1 Francisco Martínez, Patricia de
A1 Martín González, Ana María
A1 Gutiérrez Fernández, Juan Carlos
AB In the present work, an extensive analysis of the putative glutathione peroxidases (GPx) of the eukaryotic microorganism model Tetrahymena thermophila is carried out. A comparative analysis with GPx present in other Tetrahymena species and other very taxonomically diverse ciliates is also performed. A majority of ciliate GPx have replaced the selenocysteine (Sec) by Cys in its catalytic center, so they can be considered as phospholipid hydroperoxide glutathione peroxidases (PHGPx). Selenocysteine insertion sequence (SECIS) elements have been detected in several ciliate GPx that do not incorporate Sec in their amino acid sequences, and conversely, in other ciliate GPx with Sec, no SECIS elements are detected. These anomalies are analyzed and discussed. From the phylogenetic analysis using the ciliate GPx amino acid sequences, the existence of extensive intraand interspecific gene duplications that produced multiple GPx isoforms in each species is inferred. The ancestral character of the selenoproteins is also corroborated. The analysis by qRT-PCR of six selected T. thermophila GPx genes has shown a quantitative differential expression between them, depending on the stressor (oxidizing agents, apoptotic inducer or metals) and the time of exposure.
PB MDPI
SN ESSN  2076-2607
YR 2020
FD 2020-07-05
LK https://hdl.handle.net/20.500.14352/6641
UL https://hdl.handle.net/20.500.14352/6641
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 24 ago 2024