RT Journal Article
T1 Campylobacter fetus Plasmid Diversity: Comparative Analysis of Fully Sequenced Plasmids and Proposed Classification Scheme
A1 Pena Fernández, Nerea
A1 Van der Graaf-van Bloois, Linda
A1 Duim, Birgitta
A1 Zomer, Aldert
A1 Wagenaar, Jaap A.
A1 Ocejo, Medelin
A1 Lavín, José Luis
A1 Collantes Fernández, Esther
A1 Hurtado, Ana
A1 Aduriz, Gorka
AB Campylobacter fetus is an animal pathogen that contains 2 mammal-associated subspecies: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv) including its biovar intermedius that exhibit different biochemical traits and differences in pathogenicity. Although plasmids are important in the horizontal transfer of antimicrobial resistance genes and virulence factors, C. fetus plasmids are understudied. Here, the closed sequences of 12 plasmids from Spanish C. fetus isolates were compared with the publicly available DNA sequences of C. fetus plasmids and other members of the Campylobacterales order. Sizes of C. fetus plasmids from Spanish isolates ranged between 4 and 50 kb and most of them (10/12) were potentially conjugative. Comparative analysis of the plasmids’ gene content revealed a close genetic relationship between the plasmids of C. fetus isolated in Spain and those from other geographical regions, while being clearly distinct from plasmids of other Campylobacter species. Furthermore, C. fetus plasmids were grouped into two main clusters regardless of their geographic location or lineage. The distribution pattern of relaxase, replicase, and single-stranded DNA binding SSB protein encoding genes showed a clustering comparable to that resulting from plasmid whole gene content analysis, suggesting its potential use for the classification of C. fetus plasmids. Most of the larger plasmids harbored mobile genetic elements. These results can help to better understand the evolutionary dynamics and pathogenic implications of C. fetus plasmids.
PB Oxford University Press
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/109344
UL https://hdl.handle.net/20.500.14352/109344
LA eng
NO Nerea Pena-Fernández, Linda van der Graaf-van Bloois, Birgitta Duim, Aldert Zomer, Jaap A Wagenaar, Medelin Ocejo, Jose Luís Lavín, Esther Collantes-Fernández, Ana Hurtado, Gorka Aduriz, Campylobacter fetus Plasmid Diversity: Comparative Analysis of Fully Sequenced Plasmids and Proposed Classification Scheme, Genome Biology and Evolution, Volume 16, Issue 10, October 2024, evae203, https://doi.org/10.1093/gbe/evae203
NO Pre2018-086113  para Nerea Pena-Fernández
NO European Commission
NO Eusko Jaurlaritza
DS Docta Complutense
RD 6 abr 2025