RT Journal Article
T1 Mesoporous Silica Nanoparticles Decorated with Carbosilane Dendrons as New Non-viral Oligonucleotide Delivery Carriers
A1 Martínez, Angel
A1 Fuentes Paniagua, Elena
A1 Baeza, Alejandro
A1 Sánchez Nieves, Javier
A1 Cicuéndez Maroto, Mónica
A1 Gómez, Rafael
A1 Mata, F. Javier de la
A1 González, Blanca
A1 Vallet Regí, María Dulce Nombre
AB A novel nanosystem based on mesoporous silica nanoparticles covered with carbosilane dendrons grafted on the external surface of the nanoparticles is reported. This system is able to transport single-stranded oligonucleotide into cells, avoiding an electrostatic repulsion between the cell membrane and the negatively charged nucleic acids thanks to the cationic charge provided by the dendron coating under physiological conditions. Moreover, the presence of the highly ordered pore network inside the silica matrix would make possible to allocate other therapeutic agents within the mesopores with the aim of achieving a double delivery. First, carbosilane dendrons of second and third generation possessing ammonium or tertiary amine groups as peripheral functional groups were prepared. Hence, different strategies were tested in order to obtain their suitable grafting on the outer surface of the nanoparticles. As nucleic acid model, a single-stranded DNA oligonucleotide tagged with a fluorescent Cy3 moiety was used to evaluate the DNA adsorption capacity. The hybrid material functionalised with the third generation of a neutral dendron showed excellent DNA binding properties. Finally, the cytotoxicity as well as the capability to deliver DNA into cells, was tested in vitro by using a human osteoblast-like cell line, achieving good levels of internalisation of the vector DNA/carbosilane dendron-functionalised material without affecting the cellular viability.
PB Wiley
SN 0947-6539
YR 2015
FD 2015-09-15
LK https://hdl.handle.net/20.500.14352/23172
UL https://hdl.handle.net/20.500.14352/23172
LA eng
NO RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)ORCID 0000-0002-9042-8865 (Alejandro Baeza)RESEARCHER ID K-8193-2014 (Alejandro Baeza)ORCID 0000-0002-0493-6071 (Blanca González Ortiz)RESEARCHER ID K-4773-2015 (Blanca González Ortiz)
NO Ministerio de Economia y Competitividad (MINECO)
NO Comunidad de Madrid
NO Agening Network of Excellence
NO Universidad Complutense de Madrid
NO UAH
NO Ministerio de Educacion
NO Instituto de Salud Carlos III
NO VI National RDi Plan
DS Docta Complutense
RD 22 jul 2024