RT Journal Article
T1 The impact of polyphenols on chondrocyte growth and survival: a preliminary report
A1 Fernández Arroyo, Salvador
A1 Huete Toral, Fernando
A1 Pérez de Lara, María Jesús
A1 Cádiz Gurrea, María de la Luz
A1 Legeai Mallet, Laurence
A1 Micol, Vicente
A1 Segura Carretero, Antonio
A1 Joven, Jorge
A1 Pintor, Jesús
AB Background: Imbalances in the functional binding of fibroblast growth factors (FGFs) to their receptors (FGFRs) have consequences for cell proliferation and differentiation that in chondrocytes may lead to degraded cartilage. The toxic, proinflammatory, and oxidative response of cytokines and FGFs can be mitigated by dietary polyphenols.Objetive: We explored the possible effects of polyphenols in the management of osteoarticular diseases using a model based on the transduction of a mutated human FGFR3 (G380R) in murine chondrocytes. This mutation is present in most cases of skeletal dysplasia and is responsible for the overexpression of FGFR3 that, in the presence of its ligand, FGF9, results in toxic effects leading to altered cellular growth.Design: Different combinations of dietary polyphenols derived from plant extracts were assayed in FGFR3 (G380R) mutated murine chondrocytes, exploring cell survival, chloride efflux, extracellular matrix (ECM) generation, and grade of activation of mitogen-activated protein kinases.Results: Bioactive compounds from Hibiscus sabdariffa reversed the toxic effects of FGF9 and restored normal growth, suggesting a probable translation to clinical requests in humans. Indeed, these compounds activated the intracellular chloride efflux, increased ECM generation, and stimulated cell proliferation. The inhibition of mitogen-activated protein kinase phosphorylation was interpreted as the main mechanism governing these beneficial effects.Conclusions: These findings support the rationale behind the encouragement of the development of drugs that repress the overexpression of FGFRs and suggest the dietary incorporation of supplementary nutrients in the management of degraded cartilage.
PB Taylor & Francis
SN 1654-6628
YR 2015
FD 2015-10-05
LK https://hdl.handle.net/20.500.14352/23176
UL https://hdl.handle.net/20.500.14352/23176
LA eng
NO En O.A. en la web del editor.Received: 30 July 2015; Revised: 8 September 2015; Accepted: 8 September 2015; Published: 5 October 2015
NO Instituto de Salud Carlos III de Madrid
NO Consejería de Innovación y Ciencia de Andalucía (España)
NO Generalitat Valenciana (España)
NO Fundación Areces (España)
NO Fundación MAGAR (España)
DS Docta Complutense
RD 10 abr 2025