RT Journal Article
T1 Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2
A1 Rodríguez Peña, José Manuel
A1 Diez-Muñiz, Sonia
A1 Bermejo, Clara
A1 Nombela, César
A1 Arroyo, Javier
AB Yeast adaptation to conditions in which cell wall integrity is compromised mainly relies on the cell wall integrity (CWI) mitogen-activated protein kinase (MAPK) pathway. Zymolyase, a mixture of cell wall-digesting enzymes, triggers a peculiar signaling mechanism in which activation of the CWI pathway is dependent on the high-osmolarity glycerol MAPK pathway. We have identified inhibitors of the principal enzyme activities present in zymolyase and tested their effect on the activation of the MAPK of the CWI pathway, Slt2/Mpk1. Eventually, only β-1,3-glucanase and protease activities were essential to elicit Slt2 activation and confer lytic power to zymolyase. Moreover, we show that the osmosensor Hkr1 is required for signaling, being the most upstream element identified to date.
PB Elsevier
SN 0014-5793
YR 2013
FD 2013-10-04
LK https://hdl.handle.net/20.500.14352/34909
UL https://hdl.handle.net/20.500.14352/34909
LA eng
NO Ministerio de Ciencia e Innovación (MICCIN)
NO Comunidad Autónoma de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 8 abr 2025