RT Journal Article
T1 Validation of a Generation 4 Phosphorus-Containing Polycationic Dendrimer for Gene Delivery Against HIV-1
A1 Briz, Veronica
A1 Serramia, María Jesús
A1 Madrid González, Ricardo
A1 Hameau, Aurélien
A1 Caminade, Anne-Marie
A1 Majoral, Jean Pierre
A1 Muñoz-Fernández, María Ángeles
AB Gene therapy, in which oligomeric genetic material is carried into cells by nano-sized gene delivery vehicles to interfere with gene expression, represents a promising approach for preventive therapy against HIV/AIDS pandemic. Herein, we evaluate the usefulness of a phosphorus-containing dendrimer G4(NH+Et2Cl-)96 as a delivery agent of ODNs and siRNAs. G4(NH+Et2Cl-)96 formed stable complexes with ODNs or siRNAs and exhibited very low cytotoxicity in Sup T1 cells or PBMC. Functional validation was performed by using specific siRNA against HIV-1 Nef, siNEF to interfere in HIV-1 replication. G4(NH+Et2Cl-)96/siNEF dendriplex showed a high efficiency in Nef silencing. Furthermore, in vitro treatment of HIV-infected PBMC with G4(NH+Et2Cl-)96/siNEF dendriplex significantly reduced the viral replication. Our results prove the usefulness of phosphorus-containing dendrimers to deliver and transfect siRNA into CD4-T cells as a potential alternative therapy in the HIV-1 infection.
PB Bentham Science Publishers
SN 0929-8673
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/98366
UL https://hdl.handle.net/20.500.14352/98366
LA eng
NO Briz, V., et al. «Validation of a Generation 4 Phosphorus-Containing Polycationic Dendrimer for Gene Delivery Against HIV-1». Current Medicinal Chemistry, vol. 19, n.o 29, pp. 5044-51. https://doi.org/10.2174/0929867311209025044.
DS Docta Complutense
RD 6 abr 2025