RT Journal Article
T1 Plasma rich in growth factors (PRGF) and leukocyte-platelet rich fibrin (L-PRF): comparative release of growth factors and biological effect on osteoblasts
A1 Baca-Gonzalez, Laura
A1 Rancán, Lisa
A1 Fernández-Tresguerres Hernández-Gil, Isabel
A1 López-Pintor Muñoz, Rosa María
A1 López-Quiles Martínez, Juan
A1 Leco Berrocal, María Isabel
A1 Torres García Denche, Jesús
AB Purpose                To compare the release of platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF-I) and interleukin 1β (IL-1β) of plasma rich in growth factors (PRGF) and leucocyte platelet-rich fibrin (L-PRF) and to evaluate their biological implication in osteoblasts.                              Methods                Blood from 3 healthy volunteers was processed into PRGF, immediate L-PRF (L-PRF 0ʹ) and L-PRF 30 min after collection (L-PRF-30ʹ) and a control group. Growth factors release were analyzed at 7 times by ELISA. Cell proliferation, collagen-I synthesis and alkaline phosphatase activity were assessed in primary cultures of human osteoblasts.                              Results                A slower controlled release of IGF-I, VEGF and PDGF was observed in the PRGF group at day 14. A higher synthesis of type I collagen was also quantified in PRGF. L-PRF released significantly higher amounts of IL-1β, that was almost absent in the PRGF.                              Conclusions                The addition of leukocytes dramatically increases the secretion of proinflammatory cytokines, which are likely to negatively influence the synthesis of type I collagen and alkaline phosphatase (ALP) by osteoblasts.
SN 2198-4034
YR 2022
FD 2022-10-03
LK https://hdl.handle.net/20.500.14352/114394
UL https://hdl.handle.net/20.500.14352/114394
LA eng
NO Baca-Gonzalez L, Serrano Zamora R, Rancan L, González Fernández-Tresguerres F, Fernández-Tresguerres I, López-Pintor RM, López-Quiles J, Leco I, Torres J. Plasma rich in growth factors (PRGF) and leukocyte-platelet rich fibrin (L-PRF): comparative release of growth factors and biological effect on osteoblasts. Int J Implant Dent. 2022 Oct 3;8(1):39. doi: 10.1186/s40729-022-00440-4. PMID: 36184700; PMCID: PMC9527267.
DS Docta Complutense
RD 5 abr 2025