RT Journal Article
T1 Cytotoxicity, uptake and accumulation of selenium nanoparticles and other selenium species in neuroblastoma cell lines related to Alzheimer’s disease by using cytotoxicity assays, TEM and single cell-ICP-MS
A1 Vicente Zurdo, David
A1 Gómez Gómez, Beatriz
A1 Romero Sánchez, Iván
A1 Rosales Conrado, Noelia
A1 León González, María Eugenia De
A1 Madrid Albarrán, María Yolanda
A2 Elsevier B.V., 
AB Alzheimer’s disease (AD) is the most prevalent neurodegenerative disease, representing 80% of the total dementia cases. The “amyloid cascade hypothesis” stablishes that the aggregation of the beta-amyloid protein (Aβ42) is the first event that subsequently triggers AD development. Selenium nanoparticles stabilized with chitosan (Ch-SeNPs) have demonstrated excellent anti-amyloidogenic properties in previous works, leading to an improvement of AD aetiology. Here, the in vitro effect of selenium species in AD model cell line has been study to obtain a better assessment of their effects in AD treatment. For this purpose, mouse neuroblastoma (Neuro-2a) and human neuroblastoma (SH-SY5Y) cell lines were used. Cytotoxicity of selenium species, such as selenomethionine (SeMet), Se-methylselenocysteine (MeSeCys) and Ch-SeNPs, has been determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry methods. Intracellular localisation of Ch-SeNPs, and their pathway through SH-SY5Y cell line, have been evaluated by transmission electron microscopy (TEM). The uptake and accumulation of selenium species by both neuroblastoma cell lines have been quantified at single cell level by single cell- Inductively Coupled Plasma with Mass Spectrometry detection (SCICP-MS), with a previous optimisation of transport efficiency using gold nanoparticles (AuNPs) ((69 ± 3) %) and 2.5 mm calibration beads ((92 ± 8) %). Results showed that Ch-SeNPs would be more readily accumulated by both cell lines than organic species being accumulation ranges between 1.2 and 89.5 fg Se cell􀀀 1 for Neuro-2a and 3.1–129.8 fg Se cell1 for SH-SY5Y exposed to 250 μM Ch-SeNPs. Data obtained were statistically using chemometric tools. These results provide an important insight into the interaction of Ch-SeNPs with neuronal cells, which could support their potential use in AD treatment.
PB Elsevier
SN 0003-2670
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/91806
UL https://hdl.handle.net/20.500.14352/91806
LA eng
NO Vicente-Zurdo D, Gómez-Gómez B, Romero-Sánchez I , Rosales-Conrado N , León-González ME , Madrid Y, Cytotoxicity, uptake and accumulation of selenium nanoparticles and other selenium species in neuroblastoma cell lines related to Alzheimer’s disease by using cytotoxicity assays, TEM and single cell-ICP-MS. Analytica Chimica Acta 2023 1249:340949
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
NO European Commission
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 25 ago 2024