RT Journal Article
T1 Prospective analysis of biomarkers associated with successful faecal microbiota transplantation in recurrent Clostridioides difficile infection
A1 Vázquez-Cuesta, Silvia
A1 Olmedo, María
A1 Kestler Hernández, Martha Jeannette
A1 Álvarez-Uría, Ana
A1 De la Villa, Sofía
A1 Alcalá, Luis
A1 Marín Arriaza, María De Las Mercedes
A1 Rodríguez-Fernández, Sara
A1 Sánchez-Martínez, Celia
A1 Muñoz García, Patricia Carmen
A1 Bouza Santiago, Emilio
A1 Reigadas, Elena
AB ObjectivesFaecal microbiota transplantation (FMT) is an established treatment for recurrent Clostridioides difficile infection (CDI). This study aimed to identify calprotectin and microbiome characteristics as potential biomarkers of FMT success.MethodsWe conducted a prospective study of patients who underwent oral FMT (single dose of 4–5 capsules) for recurrent CDI (January 2018 to December 2022). Samples were collected at three time points: at CDI diagnosis, within 24 hours before FMT administration, and 30 days post-FMT. Calprotectin levels were assessed and the V4 region of the 16S rRNA gene was sequenced to analyse the microbiota composition. Sequencing data analysis and statistical analysis were performed using MOTHUR and R.ResultsNinety-seven patients underwent FMT (totalling 105 procedures). A total of 221 samples were processed, including 21 donor samples, 24 capsule contents, and 176 patient faecal samples (39 at diagnosis, 63 pre-FMT, and 74 post-FMT). FMT achieved an overall success rate of 85.1% (86/101 cases). The abundance of Bacteroides, Ruminococcus, Megamonas, and certain Prevotella operational taxonomic units was significantly higher in capsules associated with 100% success compared with less effective capsules. FMT engraftment was observed in 95% of patients with favourable outcomes versus 62% of those with recurrences (p 0.006). Additionally, a negative correlation was found between calprotectin levels and specific microbial genera, suggesting an association with successful outcomes.DiscussionThis study highlights differences in the evolution of faecal microbiota, bacterial engraftment, and inflammation markers (e.g. calprotectin) between patients with varying FMT outcomes. Potential biomarkers for successful FMT were identified, providing valuable insights for optimizing FMT strategies.
PB Elsevier
SN 1198-743X
YR 2025
FD 2025-01-25
LK https://hdl.handle.net/20.500.14352/134123
UL https://hdl.handle.net/20.500.14352/134123
LA eng
NO Vázquez-Cuesta S, Olmedo M, Kestler M, Álvarez-Uría A, De la Villa S, Alcalá L, et al. Prospective analysis of biomarkers associated with successful faecal microbiota transplantation in recurrent Clostridioides difficile infection. Clin Microbiol Infect. 2025;31(7):1187-1195. doi:10.1016/j.cmi.2025.01.026
DS Docta Complutense
RD 8 abr 2026