RT Journal Article
T1 Multifunctional antibiotic- and zinc-containing mesoporous bioactive glass scaffolds to fight bone infection.
A1 Heras, Clara
A1 Jiménez Holguín, Javier
A1 Doadrio Villarejo, Antonio Luis
A1 Vallet Regí, María Dulce Nombre
A1 Sánchez Salcedo, Sandra
A1 Salinas Sánchez, Antonio Jesús
AB Bone regeneration is a clinical challenge which requires multiple approaches. Sometimes, it also includes the development of osteogenic and antibacterial biomaterials to treat the emergence of possible infection processes arising from surgery. This study evaluates the antibacterial properties of gelatin-coated meso-macroporous scaffolds based on the bioactive glass 80%SiO2–15%CaO–5%P2O5 (mol-%) before (BL-GE) and after being doped with 4% of ZnO (4ZN-GE) and loaded with both saturated and the minimal inhibitory concentrations of one of the antibiotics: levofloxacin (LEVO), vancomycin (VANCO), rifampicin (RIFAM) or gentamicin (GENTA). After physical-chemical characterization of materials, release studies of inorganic ions and antibiotics from the scaffolds were carried out. Moreover, molecular modelling allowed determining the electrostatic potential density maps and the hydrogen bonds of antibiotics and the glass matrix. Antibacterial in vitro studies (in planktonic, inhibition halos and biofilm destruction) with S. aureus and E. coli as bacteria models showed a synergistic effect of zinc ions and antibiotics. The effect was especially noticeable in planktonic cultures of S. aureus with 4ZN-GE scaffolds loaded with VANCO, LEVO or RIFAM and in E. coli cultures with LEVO or GENTA. Moreover, S. aureus biofilms were completely destroyed by 4ZN-GE scaffolds loaded with VANCO, LEVO or RIFAM and the E. coli biofilm total destruction was accomplished with 4ZN-GE scaffolds loaded with GENTA or LEVO. This approach could be an important step in the fight against microbial resistance and provide needed options for bone infection treatment.
PB Elsevier
SN 1742-7061
YR 2020
FD 2020-07-24
LK https://hdl.handle.net/20.500.14352/6421
UL https://hdl.handle.net/20.500.14352/6421
LA eng
NO Heras, C., Jiménez Holguín, J., Doadrio Villarejo, A. L. et al. «Multifunctional Antibiotic- and Zinc-Containing Mesoporous Bioactive Glass Scaffolds to Fight Bone Infection». Acta Biomaterialia, vol. 114, septiembre de 2020, pp. 395-406. DOI.org (Crossref), https://doi.org/10.1016/j.actbio.2020.07.044.
NO RESEARCHER ID D-8709-2017 (Antonio Doadrio Villarejo)ORCID 0000-0001-8380-4781 (Antonio Doadrio Villarejo)RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo)ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)RESEARCHER ID M-3316-2014 (Antonio Salinas Sánchez)ORCID 0000-0002-8408-3389 (Antonio Salinas Sánchez)
NO Unión Europea. Horizonte 2020
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional
DS Docta Complutense
RD 28 may 2026