RT Journal Article
T1 The ribotoxin -sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes
A1 Olombrada, Miriam
A1 Peña, Cohue
A1 Rodríguez Galán, Olga
A1 Klingauf Nerurkar, Purnima
A1 Portugal Calisto, Daniela
A1 Oborská Oplová, Michaela
A1 Altvater, Martin
A1 Gavilanes, José G.
A1 Martínez del Pozo, Álvaro
A1 Cruz, Jesús de la
A1 García Ortega, Lucía
A1 Govind Panse, Vikram
AB The ribotoxin -sarcin belongs to a family of ribonucleases that cleave the sarcin/ricin loop (SRL), a critical functional rRNA element within the large ribosomal subunit (60S), thereby abolishing translation. Whether -sarcin targets the SRL only in mature 60S subunits remains unresolved. Here, we show that, in yeast, -sarcin can cleave SRLs within late 60S pre-ribosomes containing mature 25S rRNA but not nucleolar/nuclear 60S pre-ribosomes containing 27S pre-rRNA in vivo. Conditional expression of -sarcin is lethal, but does not impede early pre-rRNA processing, nuclear export and the cytoplasmic maturation of 60S pre-ribosomes. Thus, SRL-cleaved containing late 60S pre-ribosomes seem to escape cytoplasmic proofreading steps. Polysome analyses revealed that SRL-cleaved 60S ribosomal subunits form 80S initiation complexes, but fail to progress to the step of translation elongation. We suggest that the functional integrity of a -sarcin cleaved SRL might be assessed only during translation.
PB Oxford university press
SN 1362-4962
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/6574
UL https://hdl.handle.net/20.500.14352/6574
LA eng
NO Unión Europea. FP7
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 3 may 2024