RT Journal Article
T1 Recapitulating solid stress on tumor on a chip for nanomedicine diffusive transport prediction
A1 Martín Asensio, Alberto
A1 Dávila, Sergio
A1 Cacheux, Jean
A1 Lindstaedt, Agnieszka
A1 Dziadosz, Alicja
A1 Witt, Darius
A1 Calero Calero, Macarena
A1 Balaz, Igor
A1 Rodríguez, Isabel
AB The characteristic mechanical forces at play within tumors include the abnormal solid and fluid stresses. These, together with the increased extracellular matrix (ECM) stiffness, are the major transport barriers affecting the nanomedicine delivery to solid tumors. Due to the elevated pressure within the tumor microenvironment, the transport of nanomedicines through the interstitial space is limited to diffusion. While this particular scenario is central for nanomedicine delivery to solid tumors, it has not been modeled in vitro before. To this end, herein, a tumor-on-a-chip microfluidic device is developed that is capable of recapitulating the solid stress scenario in tumors. This is achieved by integrating a pneumatic actuation to apply compression to the enclosed hydrogel ECM filling medium. Transport studies of model nanoparticles (NPs) across this medium are performed to determine their diffusion. For these NPs, it is demonstrated that their transport is drastically reduced by 65% due to the compression of the ECM gel matrix, reducing its pore size, with only an applied pressure of  4 Pa. The results obtained show that the actuated tumor-on-a-chip device can be used to evaluate the diffusive penetration capability of nanomedicines within a mechanical-constrained microenvironment such that of tumors.
PB Wiley
SN 2699-9307
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/92695
UL https://hdl.handle.net/20.500.14352/92695
LA eng
NO Martín-Asensio, Alberto, et al. «Recapitulating Solid Stress on Tumor on a Chip for Nanomedicine Diffusive Transport Prediction». Advanced NanoBiomed Research, vol. 3, n.o 6, junio de 2023, p. 2200164. https://doi.org/10.1002/anbr.202200164.
NO European Commission
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 6 abr 2025