RT Journal Article
T1 In vivo behavior in rabbit radius bone defect of scaffolds based on nanocarbonate hydroxyapatite
A1 García Lamas, Lorena
A1 Peña López, Juan
A1 Román Zaragoza, Jesús
A1 Cabañas Criado, María Victoria
A1 Bravo Giménez, Beatriz
A1 Jiménez Díaz, Verónica
A1 Sánchez Salcedo, Sandra
A1 Jiménez Holguín, Javier
A1 Abella García, Mónica
A1 Desco, Manuel
A1 Lozano Borregón, Daniel
A1 Cecilia López, David
A1 Salinas Sánchez, Antonio Jesús
AB Bone defects treatment may require the use of biomaterials that behave as a support and promote bone regeneration. Limitations associated with the use of autografts and allografts make it necessary to design new synthetic bone substitutes. Some of the most promising biomaterials currently under investigation are based on nanocarbonate hydroxyapatite (nCHA). In this study, we studied the bone-inducing capacity of nCHA-based scaffolds alone (SAG) and enriched with osteostatin (SAGO) or with bone marrow aspirate(SAGB) after implantation for 12 weeks in a 15-mm long critical defect performed in the radius of New Zealand rabbits. Bone formation obtained was compared with a group with the unfilled defect (CE), as control group, and other with the defect filed with iliac crest autograft (GS), as gold standard. X-ray follow-up was performed at 2, 4, 6 and 12 weeks and μCT and histological studies at 12 weeks. The radiological results showed a greater increment in bone formation in the GS group (75%–100%), followed by the SAG and SAGB groups (50%–75%). μCT results showed an increase of bone volume/tissue volume values in GS group followed by SAG and SAGB groups (0.53, 0.40, and 0.31 respectively) compared with CE group (0.26). Histological results showed limited resorption of the nCHA scaffolds and partial osseointegration in the SAG and SAGB groups. However, in the SAGO group, the presence of connective tissue encapsulating the scaffold was detected. In SAG, SAGB, and increase of bone formation were observed compared with CE group, but less than the GS group. Thus, the investigated materials represent a significant advance in the design of synthetic materials for bone grafting, but further studies are needed to bring their in vivo behavior closer to autograft, the gold standard.
SN 1552-4973
YR 2024
FD 2024-01-12
LK https://hdl.handle.net/20.500.14352/101661
UL https://hdl.handle.net/20.500.14352/101661
LA eng
NO García‐Lamas L, Peña J, Roman J, Cabañas V, Bravo‐Giménez B, Jiménez‐Díaz V, et al. In vivo behavior in rabbit radius bone defect of scaffolds based on nanocarbonate hydroxyapatite. J Biomed Mater Res 2024;112:e35391. https://doi.org/10.1002/jbm.b.35391.
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 7 abr 2025