RT Journal Article
T1 HvPap-1 C1A Protease and HvCPI-2 Cystatin Contribute to Barley Grain Filling and Germination
A1 Díaz Mendoza, María Mercedes
A1 Dominguez-Figueroa, Jose
A1 Velasco-Arroyo, Blanca
A1 Cambra, Ines
A1 Gonzalez-Melendi, Pablo
A1 Lopez-Gonzalvez, Angeles
A1 Garcia, Antonia
A1 Hensel, Goetz
A1 Kumlehn, Jochen
A1 Diaz, Isabel
A1 Martinez, Manuel
AB Proteolysis is an essential process throughout the mobilization of storage proteins in barley (Hordeum vulgare) grains during germination. It involves numerous types of enzymes, with C1A Cys proteases the most abundant key players. Manipulation of the proteolytic machinery is a potential way to enhance grain yield and quality, and it could influence the mobilization of storage compounds along germination. Transgenic barley plants silencing or over-expressing the cathepsin F-like HvPap-1 Cys protease show differential accumulation of storage molecules such as starch, proteins, and free amino acids in the grain. It is particularly striking that the HvPap-1 artificial microRNA lines phenotype show a drastic delay in the grain germination process. Alterations to the proteolytic activities in the over-expressing and knock-down grains associated with changes in the level of expression of several C1A peptidases were also detected. Similarly, down-regulating cystatin Icy-2, one of the proteinaceous inhibitors of the cathepsin F-like protease, also has important effects on grain filling. However, the ultimate physiological influence of manipulating a peptidase or an inhibitor cannot be always predicted, since the plant tries to compensate the modified proteolytic effects by modulating the expression of some other peptidases or their inhibitors.
PB Oxford University Press
SN 0032-0889
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/95782
UL https://hdl.handle.net/20.500.14352/95782
LA eng
NO Mercedes Diaz-Mendoza, Jose D. Dominguez-Figueroa, Blanca Velasco-Arroyo, Ines Cambra, Pablo Gonzalez-Melendi, Angeles Lopez-Gonzalvez, Antonia Garcia, Goetz Hensel, Jochen Kumlehn, Isabel Diaz, Manuel Martinez, HvPap-1 C1A Protease and HvCPI-2 Cystatin Contribute to Barley Grain Filling and Germination, Plant Physiology, Volume 170, Issue 4, April 2016, Pages 2511–2524, https://doi.org/10.1104/pp.15.01944
NO This work was financially supported by the Ministerio de Economia y Competitividad of Spain (projectsAGL2011-23650and BIO2014-53508-R)and European Commission FP7Marie Curie action CoFund program.
NO Ministerio de Economía y Competitividad (España)
NO European Commission
DS Docta Complutense
RD 9 abr 2025