RT Journal Article
T1 Support Enzyme Loading Influences the Effect of Aldehyde Dextran Modification on the Specificity of Immobilized Ficin for Large Proteins
A1 Siar, El Hocine
A1 Abellanas Pérez, Pedro
A1 Rocha Martín, Javier
A1 Fernández Lafuente, Roberto
AB It has been reported that the modification of immobilized glyoxyl–ficin with aldehyde dextran can promote steric hindrances that greatly reduce the activity of the immobilized protease against hemoglobin, while the protease still maintained a reasonable level of activity against casein. In this paper, we studied if this effect may be different depending on the amount of ficin loaded on the support. For this purpose, both the moderately loaded and the overloaded glyoxyl–ficin biocatalysts were prepared and modified with aldehyde dextran. While the moderately loaded biocatalyst had a significantly reduced activity, mainly against hemoglobin, the activity of the overloaded biocatalyst was almost maintained. This suggests that aldehyde dextran was able to modify areas of the moderately loaded enzyme that were not available when the enzyme was overloaded. This modification promoted a significant increase in biocatalyst stability for both biocatalysts, but the stability was higher for the overloaded biocatalyst (perhaps due to a combination of inter- and intramolecular crosslinking).
PB MDPI
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/112286
UL https://hdl.handle.net/20.500.14352/112286
LA eng
NO Siar, E. H., Abellanas-Perez, P., Rocha-Martin, J., & Fernandez-Lafuente, R. (2024). Support Enzyme Loading Influences the Effect of Aldehyde Dextran Modification on the Specificity of Immobilized Ficin for Large Proteins. Molecules, 29(15). https://doi.org/10.3390/MOLECULES29153674
NO Agencia Estatal de Investigación (España)
DS Docta Complutense
RD 10 abr 2025