RT Journal Article
T1 A next-generation sequencing approach for the detection of mixed species in canned tuna
A1 Klapper, Regina
A1 Velasco, Amaya
A1 Döring, Maik
A1 Schröder, Ute
A1 Sotelo, Carmen G.
A1 Brinks, Erik
A1 Muñoz Colmenero, Ana Marta
AB Tuna cans are relevant seafood products for which mixtures of different tuna species are not allowed according to European regulations. In order to support the prevention of food fraud and mislabelling, a next-generation sequencing methodology based on mitochondrial cytochrome b and control region markers has been tested. Analyses of defined mixtures of DNA, fresh tissue and canned tissue revealed a qualitative and, to some extent, semiquantitative identification of tuna species. While the choice of the bioinformatic pipeline had no influence in the results (p = 0.71), quantitative differences occurred depending on the treatment of the sample, marker, species, and mixture (p < 0.01). The results revealed that matrix-specific calibrators or normalization models should also be used in NGS. The method represents an important step towards a semiquantitative method for routine control of this analytically challenging food matrix. Tests of commercial samples uncovered mixed species in some cans, being not in compliance with EU regulations.
PB Elsevier
SN 2590-1575
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/110043
UL https://hdl.handle.net/20.500.14352/110043
LA eng
NO Klapper, R., Velasco, A., Döring, M., Schröder, U., Sotelo, C. G., Brinks, E., & Muñoz-Colmenero, M. (2023). A next-generation sequencing approach for the detection of mixed species in canned tuna. Food Chemistry: X, 17. https://doi.org/10.1016/J.FOCHX.2023.100560
NO This work was carried out with funding from the EU Interreg Atlantic Area programme, project “SEATRACES”, number EAPA_87/2016.
NO European Commission
DS Docta Complutense
RD 25 mar 2026