RT Journal Article
T1 Comparison of the Effect of Melatonin Treatment before and after Brain Ischemic Injury in the Inflammatory and Apoptotic Response in Aged Rats
A1 Rancán, Lisa
A1 Paredes Royano, Sergio Damián
A1 García Martín, M. Cruz
A1 González, Pablo
A1 Rodríguez-Bobada, Cruz
A1 Calvo Soto, Mario
A1 Hyacinthe, Bryan
A1 Vara Ameigeiras, Elena Mª
A1 Fernández-Tresguerres Hernández, Jesús Ángel
A1 Vara Ameigeiras, Elena María
AB Aging is associated with an increase in stroke risk. Melatonin, a potent free radical scavenger and broad spectrum antioxidant, has been shown to counteract inflammation and apoptosis in brain injury. However, little is known on the possible protective effects of melatonin in aged individuals affected by brain ischemia. Also, using melatonin before or after an ischemic stroke may result in significantly different molecular outcomes. The objective of the present study was to compare the effects of pre-ischemia vs. post-ischemia melatonin administration in an ischemic lesion in the cortex and hippocampus of senescent Wistar rats. An obstruction of the middle cerebral artery (MCA) to 18-month-old animals was performed. In general, animals treated with melatonin from 24 h prior to surgery until 7 days after the surgical procedure (PrevT) experienced a significant decrease in the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), glial fibrillary acidic protein (GFAP), Bcl-2-associated death promoter (BAD), and Bcl-2-associated X protein (BAX) in both cortex and hippocampus, while hippocampal levels of sirtuin 1 (SIRT1) and B-cell lymphoma 2 (Bcl-2) increased. Treatment of animals with melatonin only after surgery (AT) resulted in similar effects, but to a lesser extent than in the PrevT group. In any case, melatonin acted as a valuable therapeutic agent protecting aged animals from the harmful effects of cerebral infarction.
PB MDPI
SN 1422-0067
YR 2018
FD 2018-07-19
LK https://hdl.handle.net/20.500.14352/12569
UL https://hdl.handle.net/20.500.14352/12569
LA eng
NO Rancán, L., Paredes Royano, S. D., García Martín, M. C. et al. «Comparison of the Effect of Melatonin Treatment before and after Brain Ischemic Injury in the Inflammatory and Apoptotic Response in Aged Rats». International Journal of Molecular Sciences, vol. 19, n.o 7, julio de 2018, p. 2097. DOI.org (Crossref), https://doi.org/10.3390/ijms19072097.
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 22 abr 2025