RT Journal Article
T1 Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis
A1 Abril, Ana G.
A1 Carrera, Mónica
A1 Böhme, Karola
A1 Barros-Velázquez, Jorge
A1 Cañas, Benito
A1 Rama, Jose Luis R.
A1 Villa, Tomás G.
A1 Calo-Mata, Pilar
AB The present work describes LC-ESI-MS/MS MS (liquid chromatography-electrospray ionization-tandem mass spectrometry) analyses of tryptic digestion peptides from phages that infect mastitis-causing Staphylococcus aureus isolated from dairy products. A total of 1933 nonredundant peptides belonging to 1282 proteins were identified and analyzed. Among them, 79 staphylococcal peptides from phages were confirmed. These peptides belong to proteins such as phage repressors, structural phage proteins, uncharacterized phage proteins and complement inhibitors. Moreover, eighteen of the phage origin peptides found were specific to S. aureus strains. These diagnostic peptides could be useful for the identification and characterization of S. aureus strains that cause mastitis. Furthermore, a study of bacteriophage phylogeny and the relationship among the identified phage peptides and the bacteria they infect was also performed. The results show the specific peptides that are present in closely related phages and the existing links between bacteriophage phylogeny and the respective Staphylococcus spp. infected.
PB MDPI
SN 2304-8158
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/7483
UL https://hdl.handle.net/20.500.14352/7483
LA eng
NO Ministerio de Economía y Competitividad
NO GAIN-Xunta de Galicia
NO Spanish AEI/EU-FEDER
DS Docta Complutense
RD 11 abr 2025