RT Journal Article
T1 Bacillus subtilis RarA Acts as a Positive RecA Accessory Protein
A1 Romero, Héctor
A1 Serrano, Ester
A1 Hernández-Tamayo, Rogelio
A1 Carrasco, Begoña
A1 Cárdenas Mastrascusa, Paula Patricia
A1 Ayora, Silvia
A1 Graumann, Peter L.
A1 Alonso, Juan C.
AB Ubiquitous RarA AAAC ATPases play crucial roles in the cellular response to blocked replication forks in pro- and eukaryotes. Here, we provide evidence that absence of RarA reduced the viability of recA, recO, and recF15 cells during unperturbed growth. The rarA gene was epistatic to recO and recF genes in response to H2O2- or MMS-induced DNA damage. Conversely, the inactivation of rarA partially suppressed the HR defectof mutants lacking end-resection (addAB, recJ, recQ, recS) or branch migration (ruvAB, recG, radA) activity. RarA contributes to RecA thread formation, that are thought to be the active forms of RecA during homology search. The absence of RarA reduced RecA accumulation, and the formation of visible RecA threads in vivo upon DNA damage. When rarA was combined with mutations in genuine RecA accessory genes, RecA accumulation was further reduced in rarA recU and rarA recX double mutant cells, and was blocked in rarA recF15 cells. These results suggest that RarA contributes to the assembly of RecA nucleoprotein filaments onto single-stranded DNA, and possibly antagonizes RecA filament disassembly.
PB Frontiers
SN 1664-302X
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/113204
UL https://hdl.handle.net/20.500.14352/113204
LA eng
NO Romero, Hector, et al. «Bacillus subtilis RarA Acts as a Positive RecA Accessory Protein». Frontiers in Microbiology, vol. 11, febrero de 2020, p. 92.  https://doi.org/10.3389/fmicb.2020.00092.
NO Ministerio de ciencia, innovación y universidades (España)
NO Deutsche Forschungsgemeinschaft (Alemania)
DS Docta Complutense
RD 4 abr 2025