RT Journal Article T1 Mapping Immune Correlates and Surfaceome Genes in BRAF Mutated Colorectal Cancers A1 Cabañas Morafraile, Esther A1 Saiz Ladera, Cristina A1 Nieto Jiménez, Cristina A1 Győrffy, Balázs A1 Nagy, Adam A1 Velasco Díez, Guillermo A1 Pérez Segura, Pedro A1 Ocaña, Alberto AB Despite the impressive results obtained with immunotherapy in several cancer types, a significant fraction of patients remains unresponsive to these treatments. In colorectal cancer (CRC), B-RafV600 mutations have been identified in 8–15% of the patients. In this work we interrogated a public dataset to explore the surfaceome of these tumors and found that several genes, such as GP2, CLDN18, AQP5, TM4SF4, NTSR1, VNN1, and CD109, were upregulated. By performing gene set enrichment analysis, we also identified a striking upregulation of genes (CD74, LAG3, HLA-DQB1, HLA-DRB5, HLA-DMA, HLA-DMB, HLA-DPB1, HLA-DRA, HLA-DOA, FCGR2B, HLA-DQA1, HLA-DRB1, and HLA-DPA1) associated with antigen processing and presentation via MHC class II. Likewise, we found a strong correlation between PD1 and PD(L)1 expression and the presence of genes encoding for proteins involved in antigen presentation such as CD74, HLA-DPA1, and LAG3. Furthermore, a similar association was observed for the presence of dendritic cells and macrophages. Finally, a low but positive relationship was observed between tumor mutational burden and neoantigen load. Our findings support the idea that a therapeutic strategy based on the targeting of PD(L)1 together with other receptors also involved in immuno-modulation, such as LAG3, could help to improve current treatments against BRAF-mutated CRC tumors. PB MDPI SN 1718-7729 YR 2023 FD 2023-02-21 LK https://hdl.handle.net/20.500.14352/103447 UL https://hdl.handle.net/20.500.14352/103447 LA eng NO Instituto de Salud Carlos III (España) NO Diputación de Albacete NO Asociación Española Contra el Cáncer NO Ministerio de Ciencia, Innovación y Universidades NO European Commission NO ELIXIR (Hungría) DS Docta Complutense RD 7 abr 2025