%0 Journal Article
%A Alonso, Mario
%A Barcia Hernández, Emilia María
%A González Matilla, Juan Francisco
%A Montejo, Consuelo
%A García García, Luis
%A Villa-Hermosilla, Mónica Carolina
%A Negro Álvarez, María Sofía Elisa
%A Fraguas Sánchez, Ana Isabel
%A Fernández Carballido, Ana María
%T Funcionalizacion of Morin-Loaded PLGA Nanoparticles with Phenylalanine Dipeptide Targeting the Brain
%D 2022
%@ 1999-4923
%U https://hdl.handle.net/20.500.14352/92431
%X Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder, with its in cidence constantly increasing. To date, there is no cure for the disease, with a need for new and effective treatments. Morin hydrate (MH) is a naturally occurring flavonoid of the Moraceae family with antioxidant and anti-inflammatory properties; however, the blood–brain barrier (BBB) prevents this flavonoid from reaching the CNS when aiming to potentially treat AD. Seeking to use the LAT-1transporter present in the BBB, a nanoparticle (NPs) formulation loaded with MH and functional ized with phenylalanine-phenylalanine dipeptide was developed (NPphe-MH) and compared to non-functionalized NPs (NP-MH). In addition, two formulations were prepared using rhodamine B (Rh-B) as a fluorescent dye (NPphe-Rh and NP-Rh) to study their biodistribution and ability to cross the BBB. Functionalization of PLGA NPs resulted in high encapsulation efficiencies for both MH and Rh-B. Studies conducted in Wistar rats showed that the presence of phenylalanine dipeptide in the NPs modified their biodistribution profiles, making them more attractive for both liver and lungs, whereas non-functionalized NPs were predominantly distributed to the spleen. Formulation NPphe-Rh remained in the brain for at least 2 h after administration.
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