RT Journal Article
T1 Penicillin Acylase from Streptomyces lavendulae andAculeacin A Acylase from Actinoplanes utahensis:Two Versatile Enzymes as Useful Tools for QuorumQuenching Processes
A1 Velasco Bucheli, Rodrigo
A1 Hormigo, Daniel
A1 Fernández-Lucas, Jesús
A1 Torres Ayuso, Pedro
A1 Alfaro Ureña, Yohana
A1 Saborido Modia, Ana
A1 Serrano Aguirre, Lara
A1 García, José Luis
A1 Ramón, Fernando
A1 Acebal Sarabia, Carmen
A1 Santos de la Sen, Antonio
A1 Arroyo Sánchez, Miguel
A1 De La Mata Riesco, Mª Isabel
AB Many Gram-negative bacteria produce N-acyl-homoserine lactones (AHLs), quorum sensing (QS) molecules that can be enzymatically inactivated by quorum quenching (QQ) processes; this approach is considered an emerging antimicrobial alternative. In this study, kinetic parameters of several AHLs hydrolyzed by penicillin acylase from Streptomyces lavendulae (SlPA) and aculeacin A acylase from Actinoplanes utahensis (AuAAC) have been determined. Both enzymes catalyze efficiently the amide bond hydrolysis in AHLs with different acyl chain moieties (with or without 3-oxo modification) and exhibit a clear preference for AHLs with long acyl chains (C12-HSL > C14-HSL > C10-HSL > C8-HSL for SlPA, whereas C14-HSL > C12-HSL > C10-HSL > C8-HSL for AuAAC). Involvement of SlPA and AuAAC in QQ processes was demonstrated by Chromobacterium violaceum CV026-based bioassays and inhibition of biofilm formation by Pseudomonas aeruginosa, a process controlled by QS molecules, suggesting the application of these multifunctional enzymes as quorum quenching agents, this being the first time that quorum quenching activity was shown by an aculeacin A acylase. In addition, a phylogenetic study suggests that SlPA and AuAAC could be part of a new family of actinomycete acylases, with a preference for substrates with long aliphatic acyl chains, and likely involved in QQ processes.
PB MDPI
SN Electronic: 2073-4344
YR 2020
FD 2020-07-01
LK https://hdl.handle.net/20.500.14352/7938
UL https://hdl.handle.net/20.500.14352/7938
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Ministerio de Economía Industria y Competitividad (MINECO)
NO Comunidad de Madrid
DS Docta Complutense
RD 8 may 2024