RT Journal Article
T1 Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment
A1 Urdinguio, Rocío G.
A1 Lopez, Virginia
A1 Bayón, Gustavo F.
A1 Diaz de la Guardia, Rafael
A1 Sierra, Marta I.
A1 García Toraño, Estela
A1 Perez, Raúl F
A1 García, María G.
A1 Carella, Antonella
A1 Pruneda, Patricia C.
A1 Prieto, Cristina
A1 Dmitrijeva, Marija
A1 Santamarina, Pablo
A1 Belmonte, Thalía
A1 Mangas, Cristina
A1 Diaconu, Elena
A1 Ferrero, Cecilia
A1 Tejedor, Juan Ramón
A1 Fernandez Morera, Juan Luis
A1 Bravo, Cristina
A1 Bueno, Clara
A1 Sanjuan Pla, Alejandra
A1 Rodriguez, Ramón M.
A1 Suarez Alvarez, Beatriz
A1 López Larrea, Carlos
A1 Bernal, Teresa
A1 Colado, Enrique
A1 Balbín, Milagros
A1 García Suarez, Olivia
A1 Chiara, Maria Dolores
A1 Sáenz de Santa María, Inés
A1 Rodríguez, Francisco
A1 Pando Sandoval, Ana
A1 Rodrigo, Luis
A1 Santos, Laura
A1 Salas, Ana
A1 Vallejo Díaz, Jesús
A1 Carrera, Ana C.
A1 Rico, Daniel
A1 Hernández López, Inmaculada
A1 Vayá, Amparo
A1 Ricart, José M.
A1 Seto, Edward
A1 Sima Teruel, Núria
A1 Vaquero, Alejandro
A1 Valledor, Luis
A1 Cañal, Maria Jesus
A1 Pisano, David
A1 Graña Castro, Osvaldo
A1 Thomas, Tim
A1 Voss, Anne K.
A1 Menéndez, Pablo
A1 Villar Garea, Ana
A1 Deutzmann, Rainer
A1 Fernandez, Agustín F.
A1 Fraga, Mario F.
AB Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally differentiated blood cells, along with functional experiments, supported a role for this histone post-translational modification in the regulation of cell differentiation and apoptosis in the hematopoietic system. Furthermore, in neutrophils, H4K16ac was enriched at specific DNA repeats. These DNA regions presented an accessible chromatin conformation and were associated with the cleavage sites that generate the 50 kb DNA fragments during the first stages of programmed cell death. Our results thus suggest that H4K16ac plays a dual role in myeloid cells as it not only regulates differentiation and apoptosis, but it also exhibits a non-canonical structural role in poising chromatin for cleavage at an early stage of neutrophil cell death.
PB Oxford University Press
SN 0305-1048
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/109874
UL https://hdl.handle.net/20.500.14352/109874
LA eng
NO Urdinguio, R. G., Lopez, V., Bayón, G. F., Diaz de la Guardia, R., Sierra, M. I., García-Toraño, E., Perez, R. F., García, M. G., Carella, A., Pruneda, P. C., Prieto, C., Dmitrijeva, M., Santamarina, P., Belmonte, T., Mangas, C., Diaconu, E., Ferrero, C., Tejedor, J. R., Fernandez-Morera, J. L., Bravo, C., … Fraga, M. F. (2019). Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment. Nucleic acids research, 47(10), 5016–5037. https://doi.org/10.1093/nar/gkz195
NO European Commission
NO Instituto de Salud Carlos III
NO Ministerio de Economía y Competitividad (España)
NO Fundación Científica de la AECC
NO Fundación Ramón Areces
NO FICYT
NO Principado de Asturias
NO Deutsche Forschungsgemeinschaft
NO FERO Foundation
NO Generalitat de Catalunya
NO Fundación Cajastur
NO Fundación Josep Carreras
NO Fundación "la Caixa"
DS Docta Complutense
RD 19 abr 2025