RT Journal Article
T1 Effects of bleaching on osteoclast activity and their modulation by osteostatin and fibroblast growth factor 2
A1 Torres-Rodriguez, Carolina
A1 Portolés Pérez, María Teresa
A1 Matesanz Sancho, María Concepción
A1 Linares, Javier
A1 Feito Castellano, María José
A1 Izquierdo Barba, Isabel
A1 Esbrit, Pedro
A1 Vallet Regí, María Dulce Nombre
AB Hypothesis: Dental bleaching with H2O2 is a common daily practice in dentistry to correct discoloration of anterior teeth. The aim of this study has been to determine whether this treatment of human teeth affects growth, differentiation and activity of osteoclast-like cells, as well as the putative modulatory action of osteostatin and fibroblast growth factor 2 (FGF-2). Experiments: Previously to the in vitro assays, structural, physical-chemical and morphological features of teeth after bleaching were studied. Osteoclast-like cells were cultured on human dentin disks, pre-treated or not with 38% H2O2 bleaching gel, in the presence or absence of osteostatin (100 nM) or FGF-2 (1 ng/ml). Cell proliferation and viability, intracellular content of reactive oxygen species (ROS), pro-inflammatory cytokine (IL-6 and TNF alpha) secretion and resorption activity were evaluated. Findings: Bleaching treatment failed to affect either the structural or the chemical features of both enamel and dentin, except for slight morphological changes, increased porosity in the most superficial parts (enamel), and a moderate increase in the wettability degree. In this scenario, bleaching produced an increased osteoclast-like cell proliferation but decreased cell viability and cytokine secretion, while it augmented resorption activity on dentin. The presence of either osteostatin or FGF-2 reduced the osteoclast-like cell proliferation induced by bleaching. FGF-2 enhanced ROS content, whereas osteostatin decreased ROS but increased TNF alpha secretion. The bleaching effect on resorption activity was increased by osteostatin, but this effect was less evident with FGF-2. Conclusions: These findings further confirm the deleterious effects of tooth bleaching by affecting osteoclast growth and function as well as different modulatory actions of osteostatin and FGF-2. (C) 2015 Elsevier Inc. All rights reserved.
PB Elsevier
SN 0021-9797
YR 2016
FD 2016-01-01
LK https://hdl.handle.net/20.500.14352/23097
UL https://hdl.handle.net/20.500.14352/23097
LA eng
NO RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Ministerio de Ciencia e Innovacion (MICINN)
NO Ministerio de Economia y Competitividad (MINECO)
NO Instituto de Salud Carlos III
NO Agening Network of Excellence
DS Docta Complutense
RD 24 ago 2024