RT Journal Article
T1 Water‐Dispersible BODIPY Multifunctionalized Silicon Oxide Nanoparticles for Glutathione Sensing
A1 Bagherpour, Saman
A1 Vázquez Pérez, Patricia
A1 Redondo Horcajo, Mariano
A1 Suárez, Teresa
A1 Plaza, José Antonio
A1 Pérez García, Lluïsa
AB Glutathione (GSH), a thiol containing small peptide, plays pivotal roles in maintaining cellular redox balance, metabolism, detoxification, and scavenging of free radicals. Aberrant GSH levels in cells and tissues are associated with various disorders, underscoring the importance of accurate GSH detection for clinical diagnosis and therapy monitoring. Several molecular probes have been designed as fluorescent-based GSH sensors. However, their water insolubility and the need of using organic cosolvents hinder their applicability on biological samples. Alternatively, nanomaterials have proven to be highly promising for boosting the precision of treatments and enhancing the accuracy of diagnosing diseases, thanks to their compatibility with biological environments and improved cell uptake. Here, the synthesis and characterization of a boron-dipyrromethene (BODIPY)-based probe (PB) are reported, incorporating a fluorescent BODIPY core, chlorine substituents for reaction with GSH, and a linking moiety for conjugation to the surface of silicon oxide nanoparticles (SONPs). Functionalized SONPs with PB are also characterized at the nanoscale using high-resolution transmission electron microscopy (HR-TEM), dynamic light scattering (DLS), Zeta potential, Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), UV–Vis absorption, and fluorescence spectroscopies, confirming the surface functionalization and water-dispersibility of functionalized SONPs with PB. GSH sensing is evaluated in aqueous solution, conjugated to SONPs, and in living cells, showing promising potential for ratiometric GSH detection.
PB Wiley
SN 0934-0866
YR 2024
FD 2024-08-12
LK https://hdl.handle.net/20.500.14352/112390
UL https://hdl.handle.net/20.500.14352/112390
LA eng
NO Bagherpour, Saman, et al. «Water‐Dispersible BODIPY Multifunctionalized Silicon Oxide Nanoparticles for Glutathione Sensing». Particle & Particle Systems Characterization, vol. 41, n.o 10, octubre de 2024, p. 2400134. DOI.org (Crossref), https://doi.org/10.1002/ppsc.202400134.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Generalitat de Catalunya
DS Docta Complutense
RD 6 abr 2025