RT Journal Article
T1 Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects
A1 Suárez Barrio, Carlota
A1 Etxebarria, Jaime
A1 Hernáez Moya, Raquel
A1 del Val Alonso, Marina
A1 Rodriguez Astigarraga, Maddalen
A1 Urkaregi, Arantza
A1 Freire, Vanesa
A1 Morales, María Celia
A1 Durán, Juan
A1 Vicario De La Torre, Marta
A1 Molina Martínez, Irene Teresa
A1 Herrero Vanrell, María Del Rocío
A1 Andollo, Noelia
AB The aim of this study is to assess if an adhesive biopolymer, sodium hyaluronate (NaHA), has synergistic effects with s-PRGF (a serum derived from plasma rich in growth factors and a blood derivative that has already shown efficacy in corneal epithelial wound healing), to reduce time of healing or posology. In vitro proliferation and migration studies, both in human corneal epithelial (HCE) cells and in rabbit primary corneal epithelial (RPCE) cultures, were carried out. In addition, we performed studies of corneal wound healing in vivo in rabbits treated with s-PRGF, NaHA, or the combination of both. We performed immunohistochemistry techniques (CK3, CK15, Ki67, ß4 integrin, ZO-1, α-SMA) in rabbit corneas 7 and 30 days after a surgically induced epithelial defect. In vitro results show that the combination of NaHA and s-PRGF offers the worst proliferation rates in both HCE and RPCE cells. Addition of NaHA to s-PRGF diminishes the re-epithelializing capability of s-PRGF. In vivo, all treatments, given twice a day, showed equivalent efficacy in corneal epithelial healing. We conclude that the combined use of s-PRGF and HaNA as an adhesive biopolymer does not improve the efficacy of s-PRGF alone in the wound healing of corneal epithelial defects.
PB MDPI
SN 1422-0067
YR 2019
FD 2019-04-03
LK https://hdl.handle.net/20.500.14352/12668
UL https://hdl.handle.net/20.500.14352/12668
LA eng
NO Suárez Barrio, C., Etxebarria, J., Hernáez Moya, R. et al. «Hyaluronic Acid Combined with Serum Rich in Growth Factors in Corneal Epithelial Defects». International Journal of Molecular Sciences, vol. 20, n.o 7, abril de 2019, p. 1655. DOI.org (Crossref), https://doi.org/10.3390/ijms20071655.
NO Universidad del País Vasco/Instituto Clínico Quirúrgico de Oftalmología
NO Fundación Vasca de Innovación e Investigación Sanitaria
DS Docta Complutense
RD 19 ene 2026