RT Journal Article
T1 Toward Liquid Biopsy: Determination of the Humoral Immune Response in Cancer Patients Using HaloTag Fusion Protein-Modified Electrochemical Bioplatforms
A1 Garranzo Asensio, María
A1 Guzmán Aránguez, Ana Isabel
A1 Povés Francés, Carmen
A1 Fernández Aceñero, María Jesús
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Domínguez Muñóz, Gemma
A1 San Frutos Llorente, Luis
A1 Rodríguez Salas, Nuria
A1 Villalba Díaz, María Teresa
A1 Pingarrón Carrazón, José Manuel
A1 Campuzano Ruiz, Susana
A1 Barderas Manchado, Rodrigo
AB Autoantibodies raised against tumor-associated antigens have shown high promise as clinical biomarkers for reliable diagnosis, prognosis, and therapy monitoring of cancer. An electrochemical disposable biosensor for the specific and sensitive determination of p53-specific autoantibodies has been developed for the first time in this work. This biosensor involves the use of magnetic microcarriers (MBs) modified with covalently immobilized HaloTag fusion p53 protein as solid supports for the selective capture of specific autoantibodies. After magnetic capture of the modified MBs onto screen-printed carbon working electrodes, the amperometric signal using the system hydroquinone/H2O2 was related to the levels of p53-autoantibodies in the sample. The biosensor was applied for the analysis of sera from 24 patients with high-risk of developing colorectal cancer and 6 from patients already diagnosed with colorectal (4) and ovarian (2) cancer. The developed biosensor was able to determine p53 autoantibodies with a sensitivity higher than that of a commercial standard ELISA using a just-in-time produced protein in a simpler protocol with less sample volume and easily miniaturized and cost-effective instrumentation.
PB American Chemical society
SN 1520-6882
YR 2016
FD 2016-12-20
LK https://hdl.handle.net/20.500.14352/18029
UL https://hdl.handle.net/20.500.14352/18029
LA eng
NO Garranzo Asensio, M., Guzmán Aránguez, A. I., Povés Francés, C. et al. «Toward Liquid Biopsy: Determination of the Humoral Immune Response in Cancer Patients Using HaloTag Fusion Protein-Modified Electrochemical Bioplatforms». Analytical Chemistry, vol. 88, n.o 24, diciembre de 2016, pp. 12339-45. DOI.org (Crossref), https://doi.org/10.1021/acs.analchem.6b03526.
NO Received: September 7, 2016Accepted: November 16, 2016Published: November 16, 2016
NO Ministerio de Economía, Comercio y Empresa (España)
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 29 sept 2024