RT Journal Article
T1 Mutations of PDS5 genes enhance TAD-like domain formation in Arabidopsis thaliana
A1 Göbel, Anna Maria
A1 Zhou, Sida
A1 Wang, Zhidan
A1 Tzourtzou, Sofia
A1 Himmelbach, Axel
A1 Zheng, Shiwei
A1 Pradillo Orellana, Mónica
A1 Liu, Chang
A1 Jiang, Hua
AB In eukaryotes, topologically associating domains (TADs) organize the genome into functional compartments. While TAD-like structures are common in mammals and many plants, they are challenging to detect in Arabidopsis thaliana. Here, we demonstrate that Arabidopsis PDS5 proteins play a negative role in TAD-like domain formation. Through Hi-C analysis, we show that mutations in PDS5 genes lead to the widespread emergence of enhanced TAD-like domains throughout the Arabidopsis genome, excluding pericentromeric regions. These domains exhibit increased chromatin insulation and enhanced chromatin interactions, without significant changes in gene expression or histone modifications. Our results suggest that PDS5 proteins are key regulators of genome architecture, influencing 3D chromatin organization independently of transcriptional activity. This study provides insights into the unique chromatin structure of Arabidopsis and the broader mechanisms governing plant genome folding.
PB Nature Research
YR 2024
FD 2024-10
LK https://hdl.handle.net/20.500.14352/111040
UL https://hdl.handle.net/20.500.14352/111040
LA eng
NO Göbel, AM., Zhou, S., Wang, Z. et al. Mutations of PDS5 genes enhance TAD-like domain formation in Arabidopsis thaliana. Nat Commun 15, 9308 (2024). https://doi.org/10.1038/s41467-024-53760-x
NO This work was supported by German Research Foundation (DFG) through grant no. INST 37/935-1 FUGG, JI347/6-1 and LI 2862/8-1, the Federal Ministry of Education and Research (BMBF) under grant No. 100489995, and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 757600).
NO European Commission
NO Federal Ministry of Education and Research (Germany)
NO German Research Foundation
DS Docta Complutense
RD 6 abr 2025