RT Journal Article
T1 Cardiac Extracellular Matrix Hydrogel Enriched with Polyethylene Glycol Presents Improved Gelation Time and Increased On-Target Site Retention of Extracellular Vesicles
A1 Gómez Cid, Lidia
A1 López Donaire, María Luisa
A1 Velasco, Diego
A1 Marín, Víctor
A1 González Tomé, María Isabel
A1 Salinas, Beatriz
A1 Cussó, Lorena
A1 García, Ángel
A1 Bravo, Susana Belén
A1 Fernández Santos, María Eugenia
A1 Elvira Martínez, Carlos María
A1 Sierra, Johanna
A1 Arroba, Ester
A1 Bañares Cañizares, Rafael
A1 Grigorian Shamagian, Lilian
A1 Fernández-Avilés Díaz, Francisco Jesús
AB Stem-cell-derived extracellular vesicles (EVs) have demonstrated multiple beneficial effects in preclinical models of cardiac diseases. However, poor retention at the target site may limit their therapeutic efficacy. Cardiac extracellular matrix hydrogels (cECMH) seem promising as drug-delivery materials and could improve the retention of EVs, but may be limited by their long gelation time and soft mechanical properties. Our objective was to develop and characterize an optimized product combining cECMH, polyethylene glycol (PEG), and EVs (EVs–PEG–cECMH) in an attempt to overcome their individual limitations: long gelation time of the cECMH and poor retention of the EVs. The new combined product presented improved physicochemical properties (60% reduction in half gelation time, p < 0.001, and threefold increase in storage modulus, p < 0.01, vs. cECMH alone), while preserving injectability and biodegradability. It also maintained in vitro bioactivity of its individual components (55% reduction in cellular senescence vs. serum-free medium, p < 0.001, similar to EVs and cECMH alone) and increased on-site retention in vivo (fourfold increase vs. EVs alone, p < 0.05). In conclusion, the combination of EVs–PEG–cECMH is a potential multipronged product with improved gelation time and mechanical properties, increased on-site retention, and maintained bioactivity that, all together, may translate into boosted therapeutic efficacy.
PB MPDI
SN 1422-0067
YR 2021
FD 2021-08-26
LK https://hdl.handle.net/20.500.14352/4842
UL https://hdl.handle.net/20.500.14352/4842
LA eng
NO Gómez Cid, L., López Donaire, M. L., Velasco, D. et al. «Cardiac Extracellular Matrix Hydrogel Enriched with Polyethylene Glycol Presents Improved Gelation Time and Increased On-Target Site Retention of Extracellular Vesicles». International Journal of Molecular Sciences, vol. 22, n.o 17, agosto de 2021, p. 9226. DOI.org (Crossref), https://doi.org/10.3390/ijms22179226.
NO Instituto de Salud Carlos III
NO Comunidad de Madrid/Fondo Europeo de Desarrollo Regional
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional
DS Docta Complutense
RD 9 abr 2025