%0 Journal Article
%A Martínez Rodrigo, Abel
%A Mas Zubiri, Alicia
%A Fernández-Cotrina, Javier
%A Belinchón-Lorenzo, Silvia
%A Orden Gutiérrez, José Antonio
%A Arias, Pablo
%A Fuente López, Ricardo De La
%A Carrión Herrero, Francisco Javier
%A Domínguez Bernal, Gustavo Ramón
%T Strength and medium-term impact of HisAK70 immunization in dogs: Vaccine safety and biomarkers of effectiveness for ex vivo Leishmania infantum infection
%D 2019
%@ 0147-9571
%U https://hdl.handle.net/20.500.14352/92568
%X HisAK70 candidates have successfully been tested in cutaneous (CL) and visceral leishmaniosis (VL) mouse models. Here, we analyse different biomarkers in dog trials after a heterologous immunization strategy with a HisAK70 candidate (plasmid DNA plus adoptive transfer of peripheral blood-derived dendritic cells (DCs) pulsed with the same pathoantigen and CpG ODN as an adjuvant) to explore the antileishmanial activity in an ex vivo canine co-culture system in the presence of Leishmania infantum parasites. In the canine model, the heterologous HisAK70 vaccine could decrease the infection index in the DC-T cell co-culture system by up to 54% after 30 days and reach almost 67% after 100 days post-immunization, respectively, compared to those obtained in the control group of dogs. The observed security and potential to fight ex vivo L. infantum infection highlight a HisAK70 heterologous immunization strategy as a promising alternative to evaluate its effectiveness against canine VL.
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