RT Journal Article
T1 Low Intensity Pulsed Ultrasounds Modulate Adipose Stem Cells Differentiation
A1 Fernández Marcelo, Tamara
A1 González Gálvez, Beatriz
A1 Fernández Millán, Elisa
A1 Calero, Alba
A1 de Lucas, Beatriz
A1 Sury, Paulina
A1 Quintana, Elena
A1 Garrido, María
A1 Arregui, Raul L.
A1 Pérez, Laura M.
AB LIPUS, low intensity pulsed ultrasounds, are considered a safe and non-invasive  tool that have been used extensively in medicine for chronic diseases. We  evaluated the effects produced by LIPUS on the physiological behaviour of mouse  and human adipose stem cells (ASCs) as well as on adipose tissue explants. LIPUS  stimulation for one minute did not affect mouse or human ASC proliferation or  apoptosis, with no evident changes in morphology or cell growth. Further,  RNA-seq analysis revealed more than 200 genes differentially expressed after  ultrasound stimulation of mouse and human ASCs. Among them, the changes in gene  expression mainly belong to the canonical pathways of stemness, energy  metabolism or chemokine signalling. On the other hand, the migration ability of  mouse and human ASCs was partially affected by the ultrasound protocol, slightly  increasing their capacity to migrate, while maintaining their stemness  properties. Major affection was detected on the adipocyte differentiation  capacity. Indeed, LIPUS-treated ASCs were unable to differentiate into mature  adipocytes and their inflammatory secretion profile was reduced in both mouse  and human ASCs. These effects were confirmed on an ex vivo model of adipose  tissue explants, demonstrating that LIPUS also provoked an anti-inflammatory  profile in the adipose tissue while reducing adipocytes differentiation and  lipids content. Overall, the strong adipogenesis blockade, the reduction of  their secretion profile and the RNA-seq analysis suggest that LIPUS-treated ASCs  may be prevented from differentiating into mature adipocytes and from  exacerbating inflammation, thus limiting their contribution to obesity.
PB Springer
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/121184
UL https://hdl.handle.net/20.500.14352/121184
LA eng
NO Fernández-Marcelo, T., Calero, A., de Lucas, B. et al. Low Intensity Pulsed Ultrasounds Modulate Adipose Stem Cells Differentiation. Stem Cell Rev and Rep (2025). https://doi.org/10.1007/s12015-025-10896-7
NO Acuerdos transformativos CRUEOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. The CNS2022-135241 project assigned to BGG has been financed by MCIN/AEI/https://doi.org/10.13039/501100011033 and by NextGene rationEU/PRTR. A.C., E.Q. and T.F-M. have a contract supported by CNS2022-135241 project. This work was also supported by the grant PID2020-116134RB-I00 (MCIN/AEI/https://doi.org/10.13039/50110 0011033/and by ERDF A way of making Europe) assigned to EFM.
NO Agencia Estatal de Investigación (España)
NO European Commission
DS Docta Complutense
RD 18 dic 2025