RT Journal Article
T1 Effects of graphene oxide and reduced graphene oxide nanomaterials on porcine endothelial progenitor cells
A1 Polo Montalvo, Alberto
A1 Cicuéndez Maroto, Mónica
A1 Casarrubios Molina, Laura
A1 Barroca, Natalia
A1 Silva, Daniela
A1 Feito Castellano, María José
A1 Díez Orejas, Rosalía María
A1 Serrano López-Terradas, María de la Concepción
A1 Marques, Paula
A1 Portolés Pérez, María Teresa
AB Graphene oxide (GO) and reduced graphene oxide (rGO) have been widely used in the field of tissue regeneration and various biomedical applications. In order to use these nanomaterials in organisms, it is imperative to possess an understanding of their impact on different cell types. Due to the potential of these nanomaterials to enter the bloodstream, interact with the endothelium and accumulate within diverse tissues, it is highly relevant to probe them when in contact with the cellular components of the vascular system. Endothelial progenitor cells (EPCs), involved in blood vessel formation, have great potential for tissue engineering and offer great advantages to study the possible angiogenic effects of biomaterials. Vascular endothelial growth factor (VEGF) induces angiogenesis and regulates vascular permeability, mainly activating VEGFR2 on endothelial cells. The effects of GO and two types of reduced GO, obtained after vacuum-assisted thermal treatment for 15 min (rGO15) and 30 min (rGO30), on porcine endothelial progenitor cells (EPCs) functionality were assessed by analyzing the nanomaterial intracellular uptake, reactive oxygen species (ROS) production and VEGFR2 expression by EPCs. The results evidence that short annealing (15 and 30 minutes) at 200 °C of GO resulted in the mitigation of both the increased ROS production and decline in VEGFR2 expression of EPCs upon GO exposure. Interestingly, after 72 hours of exposure to rGO30, VEGFR2 was higher than in the control culture, suggesting an early angiogenic potential of rGO30. The present work reveals that discrete variations in the reduction of GOmay significantly affect the response of porcine endothelial progenitor cells.
SN 2040-3364
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/95090
UL https://hdl.handle.net/20.500.14352/95090
LA eng
NO Polo-Montalvo, Alberto, et al. «Effects of Graphene Oxide and Reduced Graphene Oxide Nanomaterials on Porcine Endothelial Progenitor Cells». Nanoscale, vol. 15, n.o 42, 2023, pp. 17173-83. https://doi.org/10.1039/D3NR03145D.
NO Portuguese Foundation for Science and Technology
NO European Commission
DS Docta Complutense
RD 24 ago 2024