RT Journal Article
T1 Sub-micro- and nano-sized polyethylene terephthalate deconstruction with engineered protein nanopores
A1 Heras Márquez, Diego
A1 Robles Martín, Ana.
A1 Amigot Sánchez, Rafael
A1 Fernández López, Laura
A1 González Alfonso, José L
A1 Roda, Sergi
A1 Alcolea Rodríguez, Víctor
A1 Heras Márquez, Diego
A1 Almendral, Diego
A1 Coscolín, Cristina
A1 Plou, Francisco J
A1 Portela, Raquel
A1 Bañares, Miguel Ángel
A1 Martínez Del Pozo, Álvaro
A1 García Linares, Sara
A1 Ferrer, Manuel
A1 Guallar, Víctor
AB The identification or design of biocatalysts to mitigate the accumulation of plastics, including sub-micro- and nano-sized polyethylene terephthalate (nPET), is becoming a global challenge. Here we computationally incorporated two hydrolytic active sites with geometries similar to that of Idionella sakaiensis PET hydrolase, to fragaceatoxin C (FraC), a membrane pore-forming protein. FraCm1/m2 could be assembled into octameric nanopores (7.0 nm high × 1.6–6.0 nm entry), which deconstructed (40 °C, pH 7.0) nPET from GoodFellow, commodities and plastic bottles. FraCm1 and FraCm2 degrade nPET by endo- and exo-type chain scission. While FraCm1 produces bis(2-hydroxyethyl) terephthalate as the main product, FraCm2 yields a high diversity of oligomers and terephthalic acid. Mechanistic and biochemical differences with benchmark PET hydrolases, along with pore and nPET dynamics, suggest that these pore-forming protein catalytic nanoreactors do not deconstruct macro-PET but are promising in nanotechnology for filtering, capturing and breaking down nPET, for example, in wastewater treatment plants.
YR 2023
FD 2023-10-23
LK https://hdl.handle.net/20.500.14352/112760
UL https://hdl.handle.net/20.500.14352/112760
LA eng
NO Robles-Martín, A., Amigot-Sánchez, R., Fernandez-Lopez, L. et al. Sub-micro- and nano-sized polyethylene terephthalate deconstruction with engineered protein nanopores. Nat Catal 6, 1174–1185 (2023). https://doi.org/10.1038/s41929-023-01048-6
NO Ministerio de Ciencia e Innovación
NO Fondo Europeo de Desarrollo Regional
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 24 abr 2025