RT Journal Article
T1 Analysis of Se and Hg biomolecules distribution and Se speciation in poorly studied protein fractions of muscle tissues of highly consumed fishes by SEC-UV-ICP-MS and HPLC-ESI-MS/MS
A1 Fernández Bautista, Tamara
A1 Gómez Gómez, Beatriz
A1 Gracia Lor, Emma
A1 Madrid Albarrán, María Yolanda
A1 Palacín García, Roberto
A1 Pérez Corona, María Teresa
AB Distribution of Se and Hg in sarcoplasmic, myofibrillar and alkali-soluble protein fractions extracted from muscle tissue of tuna, swordfish and salmon (wild vs. farmed) is investigated for the first time. SEC-UV-ICP-MS analyses revealed that Se and Hg are mostly bound to proteins of 2–12 kDa and up to 574 kDa, respectively. Moreover, Se and Hg appeared associated to proteins of same molecular weight, evidencing that Se–Hg interaction may occur at the level of the fish tissue evaluated. Important differences were found between farmed and wild salmon, suggesting the effect of the type of feed and growing conditions on Se and Hg content and their distribution through protein fractions. Finally, Se speciation studies performed by HPLC-ICP-MS and confirmed by HPLC-ESIMS/MS showed SeMeSeCys as the only Se specie found in soluble, sarcoplasmic, myofibrillar and alkali-soluble proteins of all fishes analysed, except in soluble proteins extracted from tuna, where SeMet was also identified.
PB Elsevier
YR 2021
FD 2021-10
LK https://hdl.handle.net/20.500.14352/130447
UL https://hdl.handle.net/20.500.14352/130447
LA eng
NO Kljakovic-Gaspic, Z, Orct, T, Sekovanic, A, Sulimanec, A, Zupan, I, Saric, MM. Consumer risks and benefits: Macro and trace elements in muscle of dominant fish species from Vrana Lake, Croatia. Environ. Chem. Ecotoxicol. 2025:7:655-670
NO Comunidad de Madrid
NO Unión Europea
NO Ministerio de Ciencia, Innovación y Universidades
DS Docta Complutense
RD 20 ene 2026