RT Journal Article
T1 Effect of Semen Collection in the Metabolite and Hormonal Content of Rabbit Seminal Plasma
A1 Álvarez Rodríguez, Manuel
A1 García Calvo, Laura
A1 Gardela, Jaume
A1 Ruiz Conca, Mateo
A1 Olvera Maneu, Sergi
A1 Martín San Juan, Adrián
A1 López Béjar, Manel
A1 Martínez Pastor, Felipe
A1 Vicente Carrillo, Alejandro
AB Blood serum (BS) and seminal plasma (SP) share a plethora of compounds that might present an individual and/or temporal concentration variation. We aimed to determine whether BS and SP concentrations of albumin, calcium, citrate, creatinine, fructose, glucose, lactate, total protein, urea, zinc, cortisol, anti-Müllerian hormone (AMH) and testosterone are related to weekly collections in New Zealand White (NZW) adult rabbit bucks. During a 12-week study, blood samples were obtained at the beginning and the end of the study period, and semen samples were taken twice a week from four NZW adult rabbit bucks, starting at 6–7 months of age. After semen collection, the sperm motility was subjectively assessed, and SP was obtained by centrifugation. BS and SP were evaluated for the above-mentioned metabolites using a Biosystems BA400 automated analyser with commercial-specific kits or enzyme immunoassay (EIA) kits, assessing the effects of the male and time of collection. In addition, a correlation analysis aimed at disclosing associations between parameters in BS and SP was performed. Male effect was not significant for BS, but it was significant for SP albumin, citrate, fructose, glucose, lactate and total protein. In addition, all the correlations in BS were positive, whereas they were more balanced in SP, being close to half of the correlations. In conclusion, variations of some metabolites (albumin, citrate, fructose, glucose, lactate and total protein) appear to be potential biomarkers for rabbit SP, although further studies should test their usefulness for sperm fertility assessment.
PB Wiley
SN 2053-1095
YR 2025
FD 2025-01-30
LK https://hdl.handle.net/20.500.14352/117697
UL https://hdl.handle.net/20.500.14352/117697
LA eng
NO Álvarez-Rodríguez, M., García-Calvo, L., Gardela, J., Ruiz-Conca, M., Olvera-Maneu, S., Martín-San Juan, A., López-Béjar, M., Martinez-Pastor, F. and Vicente-Carrillo, A. (2025), Effect of Semen Collection in the Metabolite and Hormonal Content of Rabbit Seminal Plasma. Vet Med Sci, 11: e70236. https://doi.org/10.1002/vms3.70236
NO Author Contributions:Manuel Álvarez-Rodríguez: conceptualization, methodology, writing–original draft preparation, project administration, supervision. Manel López-Béjar: conceptualization, methodology, writing–review and editing, supervision. Felipe Martinez-Pastor: conceptualization, methodology, writing–review and editing, supervision. Alejandro Vicente-Carrillo: conceptualization, methodology, writing–original draft preparation. Laura García-Calvo: methodology, writing–original draft preparation. Jaume Gardela: methodology, writing–review and editing. Mateo Ruiz-Conca: methodology, writing–review and editing. Sergi Olvera-Maneu: methodology, writing–review and editing. Adrián Martín-San Juan: methodology, writing–review and editing.
NO Ministerio de Ciencia e Innovación (España)
NO European Commission
DS Docta Complutense
RD 7 abr 2025