RT Journal Article
T1 MC3T3-E1 pre-osteoblast response and differentiation after graphene oxide nanosheet uptake
A1 Cicuéndez Maroto, Mónica
A1 Silva, V.S.
A1 Hortigüela, M.J.
A1 Matesanz, M.C.
A1 Vila Gonzalo, María
A1 Portolés Pérez, María Teresa
AB Nano-graphene oxide (GO) and its functionalized derivatives have aroused a great interest for drug delivery, tissue engineering and photothermal cancer therapy, but their biocompatibility has not yet been fully assessed. The aim of the present study was to evaluate the proliferation and differentiation of MC3T3-E1 pre-osteoblasts after the uptake of GO nanosheets (c.a. 400nm), functionalized with poly(ethylene glycol-amine) (PEG) and labelled with fluorescein isothiocyanate (FITC). Significant proliferation decrease and apoptosis increase were observed 3days after incorporation of FITC-PEG-GO by MC3T3-E1 cells. However, alterations on healthy pre-osteoblast differentiation into cells exhibiting osteoblast phenotype were not observed, as they showed normal alkaline phosphatase levels and matrix mineralization 12days after nanosheet uptake. The results suggest that 40μg/mL concentrations of these nanosheets would not affect the differentiation of healthy pre-osteoblasts, thus these PEG-GO nanosheets have potential to be used for biomedical applications after their internalization, as the induction of local hyperthermia on bone cancer.
PB Elsevier
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/96524
UL https://hdl.handle.net/20.500.14352/96524
LA eng
NO Cicuéndez M, Silva VS, Hortigüela MJ, Matesanz MC, Vila M, Portolés MT. MC3T3-E1 pre-osteoblast response and differentiation after graphene oxide nanosheet uptake. Colloids Surf B Biointerfaces. 2017 Oct 1;158:33-40. doi: 10.1016/j.colsurfb.2017.06.019. Epub 2017 Jun 20. PMID: 28662392.
NO Spanish Ministerio de Economía y Competitividad
DS Docta Complutense
RD 23 abr 2025