RT Journal Article T1 Effect of thermal liquefaction on quality, chemical composition and antibiofilm activity against multiresistant human pathogens of crystallized eucalyptus honey A1 Villacrés Granda, Irina A1 Proaño, Adrián A1 Coello, Dayana A1 Debut, Alexis A1 Vizuete, Karla A1 Ballesteros Redondo, María Isabel A1 Granda Albuja, Genoveva A1 Rosero Mayanquer, Hugo A1 Battino, Maurizio A1 Giampieri, Francesca A1 Álvarez Suárez, José M. AB Thermal liquefaction is a conventional method used by beekeepers to liquefy crystallized honey. However, an abusive use of heat may affect its quality, chemical composition and bioactivity. The purpose of this study was to investigate the effect of thermal liquefaction on the quality, chemical composition and antibiofilm properties of eucalyptus honey. Thermal liquefaction (at 45 and 60 ◦C) did not affect the honey’s quality; however, a significant reduction in the reducing capacity, total phenolic content and hydrogen peroxide content was observed. At 60 ◦C, a significant reduction in the honey’s ability to inhibit biofilm formation was observed in Pseudomonas aeruginosa, as well as a reduction in its ability to remove preformed biofilms in both Staphylococcus aureus and Pseudomonas aeruginosa. Structural changes in biofilm architecture caused by honey were not affected by thermal treatment. Therefore, we recommend liquefaction at 45 ◦C as the most convenient for honey liquefaction without affecting its characteristics. PB Elsevier SN 0308-8146 YR 2021 FD 2021-07-02 LK https://hdl.handle.net/20.500.14352/4817 UL https://hdl.handle.net/20.500.14352/4817 LA eng NO Universidad de Las Americas (UDLA), Quito, Ecuador DS Docta Complutense RD 16 may 2024