RT Journal Article
T1 Mycoflora isolation and molecular characterization of Aspergillus and Fusarium species in Tunisian cereals
A1 Jedidi, Ines
A1 Soldevilla, Carlos
A1 Lahouar, Amani
A1 Marín, Patricia
A1 González Jaén, María Teresa
A1 Said, Salem
AB Wheat, barley and maize are the mainly consumed cereals in Tunisia. This study aimed to determine the mycoflora of these cereals with special focus on the mycotoxigenic Aspergillus and Fusarium species. Freshly harvested samples and other stored samples of each type of cereal (31 and 34 samples, respectively) were collected in Tunisia and cultured for fungal isolation and identification. Identification of fungal genera was based on morphological features. Aspergillus and Fusarium species were identified by species specific PCR assays complemented with DNA sequencing. Alternaria (70.83%), Eurotium (62.50%), Aspergillus (54.17%) and Penicillium (41.67%) were the most frequent fungi isolated from wheat. Penicillium (75%), Aspergillus (70%), Eurotium (65%) and Alternaria (65%) were the most frequently recovered genera from barley. The predominant genera in maize were Aspergillus(76.19%), Eurotium (42.86%), and Penicillium (38.09%). Aspergilllus, Penicillium, Fusarium and Alternaria were detected in both stored and freshly harvested grain samples. The frequencies of contamination with Aspergillus, Fusarium and Alternaria were higher in freshly harvested samples, whereas Penicillium species were more frequent in stored samples. The predominant Aspergillus species detected were A. flavus and A. niger. The Fusarium species detected were F. equiseti, F. verticillioides, F. nygamai, and F. oxysporum. This study suggested the potential risk for Aflatoxins and, to a lesser extent, for Ochratoxin A in Tunisian cereals. This is the first survey about mycoflora associated with wheat, barley and maize in Tunisia.
PB Science Direct
SN 1319-562X
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/18456
UL https://hdl.handle.net/20.500.14352/18456
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Tunisian Ministry of Higher Education and Scientific Research
DS Docta Complutense
RD 2 oct 2024