RT Journal Article
T1 Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle
A1 Juan Ferré, Lucía De
A1 Álvarez Sánchez, Julio
A1 Aranaz Martín, Alicia
A1 Rodríguez Bertos, Antonio Manuel
A1 Romero Martínez, Beatriz
A1 Bezos Garrido, Javier
A1 Mateos García, Ana Isabel
A1 Domínguez Rodríguez, Lucas José
AB Molecular characterization of Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis) isolates classifies them into three groups: cattle or Type II, sheep or Type I, and intermediate or Type III. To avoid problems associated with characterization of extremely slow growth strains, PCR-based techniques that divide the M. a. paratuberculosis strains in two main groups (cattle or Type II, and sheep or Types I/III) can be performed. The objectives of this study were to characterize the M. a. paratuberculosis isolates identified by different PCR-based tests (IS1311-PCR and restriction endonuclease analysis, PCR test based on a DNA sequence difference, and a PCR aimed at three Type I-specific loci), and to determine the clinical and epidemiological implications of Types I/III M. a. paratuberculosis strains in livestock. One hundred and fifty-eight M. a. paratuberculosis strains from domestic ruminants were analyzed. One hundred and six M. a. paratuberculosis isolates (61 from goats and 45 from cattle) were classified as Type II strains; and 52 (29 from cows, 20 from goats, and three from sheep) were included in the Types I/III. The Types I/III M. a. paratuberculosis strains were associated to Spanish native breeds. The majority of these animals had not been in direct or indirect contact with sheep flocks infected with M. a. paratuberculosis. This fact should be taken into account when implementing paratuberculosis control programs.
PB Elsevier
SN 0378-1135
YR 2006
FD 2006
LK https://hdl.handle.net/20.500.14352/93523
UL https://hdl.handle.net/20.500.14352/93523
LA eng
NO de Juan, L., Alvarez, J., Aranaz, A., Rodríguez, A., Romero, B., Bezos, J., Mateos, A., & Domínguez, L. (2006). Molecular epidemiology of Types I/III strains of Mycobacterium avium subspecies paratuberculosis isolated from goats and cattle. Veterinary microbiology, 115(1-3), 102–110. https://doi.org/10.1016/j.vetmic.2006.01.008
NO * Corresponding author. alaranaz@vet.ucm.es (A. Aranaz).2006 Elsevier B.V. All rights reserved.Financiación: This work was funded by the European Commission (QLRT-2000-01420) and by the Spanish Ministry of Agriculture, Fisheries and Food (MAPA)
NO European Commission
NO Ministerio de Agricultura, Pesca y Alimentación (España)
DS Docta Complutense
RD 18 mar 2026