RT Journal Article
T1 Apoptosis-related proteins are potential markers of neonatal hypoxic-ischemic encephalopathy (HIE) injury
A1 Hernández Jiménez, Macarena
A1 Sacristan, Silvia
A1 Morales, Carmen
A1 García Villanueva, Mercedes
A1 García Fernández, Eugenia
A1 Alcázar, Alberto
A1 González, Víctor M.
A1 Martín, Elena
AB Neonatal hypoxic-ischemic encephalopathy (HIE) causes high mortality and long-term morbidity rates. The magnitude of the neuronal damage depends on the duration and severity of the initial insult combined with the deleterious effects of reperfusion and apoptosis. Currently, a diagnosis of HIE is based largely on the neurological and histological findings. Therefore, the aim of this study was to identify apoptosis-related proteins that might serve as potential markers of HIE injury. As an initial step toward reaching this objective, we analyzed changes in protein levels in an in vitro model of hypoxia using antibody arrays, and we have identified changes in the expression level of two proteins involved in apoptosis, Smac-DIABLO and cathepsin D. We obtained brain sections from eight neonatal HIE patients and performed histological staining, TUNEL assays and Smac-DIABLO and cathepsin D immunolocalization. Our results revealed a high number of TUNEL-positive cells, including neurons, astrocytes and ependymal cells, in the various regions that were analyzed. Interestingly, many of the areas that were positive for TUNEL staining did not appear to be damaged in the histological evaluation. In addition, using immunostaining, we found that Smac-DIABLO and cathepsin D had the same regional distribution pattern. Taken together, these findings indicate that these two proteins could serve as markers to identify injured regions that might not to be detectable using histological observations alone.
PB Elsevier
SN 0304-3940
YR 2014
FD 2014-01
LK https://hdl.handle.net/20.500.14352/113264
UL https://hdl.handle.net/20.500.14352/113264
LA eng
NO Macarena Hernández-Jiménez, Silvia Sacristán, Carmen Morales, Mercedes García-Villanueva, Eugenia García-Fernández, Alberto Alcázar, Víctor M. González, M. Elena Martín, Apoptosis-related proteins are potential markers of neonatal hypoxic–ischemic encephalopathy (HIE) injury, Neuroscience Letters, Volume 558, 2014, Pages 143-148, ISSN 0304-3940, https://doi.org/10.1016/j.neulet.2013.11.019.
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 19 dic 2025