RT Journal Article T1 Phenotypic and genetic evaluations of biogenic amine production by lactic acid bacteria isolated from fish and fish products A1 Muñoz Atienza, Estefanía A1 Landeta, G. A1 de las Rivas, B. A1 Gómez Sala, Beatriz A1 Muñoz, R. A1 Hernández Cruza, Pablo Elpidio A1 Cintas Izarra, Luis Miguel A1 Herranz Sorribes, Carmen AB In this work, biogenic amine production (histamine, tyramine and putrescine) by a collection of 74 lactic acid bacteria of aquatic origin has been investigated by means of amino acid decarboxylation by growth on decarboxylase differential medium, biogenic amine detection by thin-layer chromatography (TLC) and decarboxylase gene detection by PCR. None of the evaluated strains showed neither production of histamine and putrescine, nor presence of the genetic determinants encoding the corresponding decarboxylase activities. However, the tyrosine decarboxylase gene (tdc) was present in all the enterococcal strains, and tyramine production was detected by TLC in all of them but Enterococcus faecium BCS59 and MV5. Analysis of the tyrosine decarboxylase operon of these strains revealed the presence of an insertion sequence upstream tdc that could be responsible for their lack of tyrosine decarboxylase activity. PB Elsevier YR 2011 FD 2011-02-26 LK https://hdl.handle.net/20.500.14352/95117 UL https://hdl.handle.net/20.500.14352/95117 LA eng NO Muñoz-Atienza, E., Landeta, G., de las Rivas, B., Gómez-Sala, B., Muñoz, R., Hernández, P. E., Cintas, L. M., & Herranz, C. (2011). Phenotypic and genetic evaluations of biogenic amine production by lactic acid bacteria isolated from fish and fish products. International journal of food microbiology, 146(2), 212–216. https://doi.org/10.1016/j.ijfoodmicro.2011.02.024 NO Ministerio de Ciencia y Tecnología NO Ministerio de Ciencia e Innovación NO Comunidad de Madrid NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) DS Docta Complutense RD 1 sept 2024