RT Journal Article
T1 Determination of the boundary lipids of sticholysins from tryptophan quenching
A1 Palacios Ortega, Juan
A1 Amigot Sánchez, Rafael
A1 García Montoya, Carmen
A1 Gorse, Ana
A1 Heras Márquez, Diego
A1 García Linares, Sara
A1 Martínez Del Pozo, Álvaro
A1 Slotte, J. Peter
AB Sticholysins are α-pore-forming toxins produced by the sea-anemone Stichodactyla helianthus. These toxins exert their activity by forming pores on sphingomyelin-containing membranes. Recognition of sphingomyelin by sticholysins is required to start the process of pore formation. Sphingomyelin recognition is coupled with membrane binding and followed by membrane penetration and oligomerization. Many features of these processes are known. However, the extent of contact with each of the different kinds of lipids present in the membrane has received little attention. To delve into this question, we have used a phosphatidylcholine analogue labeled at one of its acyl chains with a doxyl moiety, a known quencher of tryptophan emission. Here we present evidence for the contact of sticholysins with phosphatidylcholine lipids in the sticholysin oligomer, and for how each sticholysin isotoxin is affected differently by the inclusion of cholesterol in the membrane. Furthermore, using phosphatidylcholine analogs that were labeled at different positions of their structure (acyl chains and headgroup) in combination with a variety of sticholysin mutants, we also investigated the depth of the tryptophan residues of sticholysins in the bilayer. Our results indicate that the position of the tryptophan residues relative to the membrane normal is deeper when cholesterol is absent from the membrane.
YR 2022
FD 2022-10-15
LK https://hdl.handle.net/20.500.14352/112282
UL https://hdl.handle.net/20.500.14352/112282
LA eng
NO Palacios-Ortega J, Amigot-Sánchez R, García-Montoya C, Gorše A, Heras-Márquez D, García-Linares S, Martínez-Del-Pozo Á, Slotte JP. Determination of the boundary lipids of sticholysins using tryptophan quenching. Sci Rep. 2022 Oct 15;12(1):17328. doi: 10.1038/s41598-022-21750-y. Erratum in: Sci Rep. 2023 Jun 8;13(1):9343. doi: 10.1038/s41598-023-36403-x. PMID: 36243747; PMCID: PMC9569322.
DS Docta Complutense
RD 7 abr 2025