RT Journal Article
T1 Protein expression knockdown in cancer cells induced by a gemini cationic lipid nanovector with histidine-based polar heads
A1 Sánchez Arribas, Natalia
A1 Martínez Negro, María
A1 Villar; Eva M., 
A1 Pérez, Lourdes
A1 Osío Barcina, José De Jesús
A1 Aicart Sospedra, Emilio
A1 Taboada, Pablo
A1 Guerrero Martínez, Andrés
A1 Junquera González, María Elena
AB A histidine-based gemini cationic lipid, which had already demonstrated its efficiency as a plasmid DNA (pDNA) nanocarrier, has been used in this work to transfect a small interfering RNA (siRNA) into cancer cells. In combination with the helper lipid monoolein glycerol (MOG), the cationic lipid was used as an antiGFP-siRNA nanovector in a multidisciplinary study. Initially, a biophysical characterization by zeta potential and agarose gel electrophoresis experiments was performed to determine the lipid effective charge and confirm siRNA compaction. The lipoplexes formed were arranged in Lalfa lamellar lyotropic liquid crystal phases with a cluster-type morphology, as cryo-transmission electron microscopy (cryo-TEM) and small-angle X-ray scattering (SAXS) studies revealed. Additionally, in vitro experiments confirmed the high gene knockdown efficiency of the lipid-based nanovehicle as detected by flow cytometry (FC) and epifluorescence microscopy, even better than that of Lipofectamine2000*, the transfecting reagent commonly used as a positive control. Cytotoxicity assays indicated that the nanovector is non-toxic to cells. Finally, using nano-liquid chromatography tandem mass spectrometry (nanoLC-MS/MS), apolipoprotein A-I and A-II followed by serum albumin were identified as the proteins with higher affinity for the surface of the lipoplexes. This fact could be beyond the remarkable silencing activity of the histidine-based lipid nanocarrier herein presented.
PB MDPI
SN 1999-4923
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/108924
UL https://hdl.handle.net/20.500.14352/108924
LA eng
NO Sánchez-Arribas N, Martínez-Negro M, Villar EM, Pérez L, Osío Barcina J, Aicart E, et al. Protein Expression Knockdown in Cancer Cells Induced by a Gemini Cationic Lipid Nanovector with Histidine-Based Polar Heads. Pharmaceutics 2020;12:791. https://doi.org/10.3390/pharmaceutics12090791.
NO Ministerio de Ciencia, Innovación y Universidades
NO Universidad Complutense de Madrid
NO Comunidad de Madrid
NO Xunta de Galicia
DS Docta Complutense
RD 7 abr 2025