RT Journal Article
T1 New specific molecular marker detects Ficopomatus enigmaticus from water eDNA before positive results of conventional sampling
A1 Muñoz Colmenero, Ana Marta
A1 Ardura Gutiérrez, Alba
A1 Clusa, Laura
A1 Miralles, Laura
A1 Gower, Fiona
A1 Zaiko, Anastasija
A1 García Vázquez, Eva
AB The tubeworm Ficopomatus enigmaticus is a highly invasive species with an increasingly expanding distribution in Europe and America. In port areas it causes damage to structures and sewage systems because it is an active reef builder. Having short generation time and high reproduction capacity it is especially important to detect the species before it is fully settled down. Here we have designed a PCR-based species-specific molecular marker within the Cytochrome Oxidase subunit I gene (COI). The new marker was validated in vitro and from field water samples obtained in southwest Bay of Biscay ports (north of Spain). The species was detected only from eDNA in Villaviciosa port, where it was not found de visu in the intertidal zone and floating structures. Adults were found in the same port one year later as confirmed taxonomically and by conventional barcoding, which confirms the utility of the new eDNA marker for early detection of F. enigmaticus. This new and cheap molecular tool could be used for routine monitoring of coastal areas, in order to early detecting this invasive species.
PB Elsevier
SN 1617-1381
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/95128
UL https://hdl.handle.net/20.500.14352/95128
LA eng
NO Muñoz-Colmenero, Marta, et al. «New Specific Molecular Marker Detects Ficopomatus Enigmaticus from Water eDNA before Positive Results of Conventional Sampling». Journal for Nature Conservation, vol. 43, junio de 2018, pp. 173-78. https://doi.org/10.1016/j.jnc.2017.12.004.
NO This study has been funded by the Spanish Ministry of Economy and Competitiveness, Grant CGL-2013-42415-R and the Principality of Asturias (Spain), Grant GRUPIN-2014-093. This is a contribution from the Marine Observatory of Asturias (OMA). Alba Ardura is recipient of a Postdoctoral Fellowship with reference Clarin-CO-FUND-PCTI-FICYT. Laura Clusa holds a PCTI Grant from the Asturias Regional Government, referenced BP14-145.
NO Ministerio de Economía y Competitividad (España)
NO Principado de Asturias
DS Docta Complutense
RD 18 abr 2025