RT Journal Article
T1 Standard methods for molecular research in Apis mellifera
A1 Evans. Jay, 
A1 Schwarz, Ryan
A1 Chen, Yan Ping
A1 Budge, Giles
A1 Cornman, Robert
A1 Rua, Pilar De la
A1 Miranda, Joachim de
A1 Foret, Sylvain
A1 Foster, Leonard
A1 Gauthier, Laurent
A1 Genersch, Elke
A1 Gisder, Sebastian
A1 Jarosch, Antje
A1 Kucharski, Robert
A1 Lopez, Dawn
A1 Lun, v
A1 Moritz, Robin
A1 Maleszka, Ryszard
A1 Muñoz Gabaldón, Irene
A1 M Alice Pinto, 
AB From studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology. With a genome sequence in hand, and much improved genetic tools, honey bees are now an even more appealing target for answering the major questions of evolutionary biology, population structure, and social organization. At the same time, agricultural incentives to understand how honey bees fall prey to disease, or evade and survive their many pests and pathogens, have pushed for a genetic understanding of individual and social immunity in this species. Below we describe and reference tools for using modern molecular-biology techniques to understand bee behaviour, health, and other aspects of their biology. We focus on DNA and RNA techniques, largely because techniques for assessing bee proteins are covered in detail in Hartfelder et al. (2013). We cover practical needs for bee sampling, transport, and storage, and then discuss a range of current techniques for genetic analysis. We then provide a roadmap for genomic resources and methods for studying bees, followed by specific statistical protocols for population genetics, quantitative genetics, and phylogenetics. Finally, we end with three important tools for predicting gene regulation and function in honey bees: Fluorescence in situ hybridization (FISH), RNA interference (RNAi), and the estimation of chromosomal methylation and its role in epigenetic gene regulation.
PB Taylor & Francis
SN 0021-8839
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/97690
UL https://hdl.handle.net/20.500.14352/97690
LA eng
NO Evans, J. D., Schwarz, R. S., Chen, Y. P., Budge, G., Cornman, R. S., De la Rua, P., … Pinto, M. A. (2013). Standard methods for molecular research in Apis mellifera. Journal of Apicultural Research, 52(4), 1–54. https://doi.org/10.3896/IBRA.1.52.4.11
NO European Commission
DS Docta Complutense
RD 5 abr 2025