RT Journal Article
T1 Nasal and Fecal Microbiota and Immunoprofiling of Infants With and Without RSV Bronchiolitis
A1 Alba Rubio, Claudio
A1 Aparicio, Marina
A1 González-Martínez, Felipe
A1 González-Sánchez, María Isabel
A1 Pérez-Moreno, Jimena
A1 Toledo del Castillo, Blanca
A1 Rodríguez Gómez, Juan Miguel
A1 Rodríguez Fernández, Rosa María
A1 Fernández Álvarez, Leonides
AB Bronchiolitis associated with the respiratory syncytial virus (RSV) is the leading cause of hospitalization among infants aged &amp;lt; 1 year. The main objective of this work was to assess the nasal and fecal microbiota and immune profiles in infants with RSV bronchiolitis, and to compare them with those of healthy infants. For this purpose, a total of 58 infants with RSV-positive bronchiolitis and 17 healthy infants (aged &amp;lt; 18 months) were recruited in this case-control study, which was approved by the Ethics Committee of the Hospital Gregorio Marañón. Nasal and fecal samples were obtained and submitted to bacterial microbiota analysis by 16S rDNA sequencing and to analysis of several immune factors related to inflammatory processes. Nasal samples in which <jats:italic>Haemophilus</jats:italic> and/or <jats:italic>Moraxella</jats:italic> accounted for &amp;gt; 20% of the total sequences were exclusively detected among infants of the bronchiolitis group. In this group, the relative abundances of <jats:italic>Staphylococcus</jats:italic> and <jats:italic>Corynebacterium</jats:italic> were significantly lower than in nasal samples from the control group while the opposite was observed for those of <jats:italic>Haemophilus</jats:italic> and <jats:italic>Mannheimia</jats:italic>. Fecal bacterial microbiota of infants with bronchiolitis was similar to that of healthy infants. Significant differences were obtained between bronchiolitis and control groups for both the frequency of detection and concentration of BAFF/TNFSF13B and sTNF.R1 in nasal samples. The concentration of BAFF/TNFSF13B was also significantly higher in fecal samples from the bronchiolitis group. In conclusion, signatures of RSV-associated bronchiolitis have been found in this study, including dominance of <jats:italic>Haemophilus</jats:italic> and a high concentration of BAFF/TNFSF13B, IL-8 and sTNF.R1 in nasal samples, and a high fecal concentration of BAFF/TNFSF13B.
PB Frontiers Media
SN 1664-302X
YR 2021
FD 2021-06-01
LK https://hdl.handle.net/20.500.14352/101042
UL https://hdl.handle.net/20.500.14352/101042
LA eng
NO Alba C, Aparicio M, González-Martínez F, González-Sánchez MI, Pérez-Moreno J, Toledo del Castillo B, Rodríguez JM, Rodríguez-Fernández R and Fernández L (2021) Nasal and Fecal Microbiota and Immunoprofiling of Infants With and Without RSV Bronchiolitis. Front. Microbiol. 12:667832. doi: 10.3389/fmicb.2021.667832
NO Ministerio de Ciencia e Innovación - PID2019-105606RB-100
DS Docta Complutense
RD 8 abr 2025