RT Journal Article
T1 Silicon as neuroprotector or neurotoxic in the human neuroblastoma SH-SY5Y cell line
A1 Garcimartín Álvarez, Alba
A1 Merino Martín, José Joaquín
A1 Santos López, Jorge Arturo
A1 López-Oliva Muñoz, María Elvira
A1 González Prieto, María Del Pilar
A1 Sánchez Muniz, Francisco José
A1 Benedí González, Juana María
AB Silicon (Si) is a trace element that has been considered to be an environmental contaminant for many years, although different studies have recently reported it is an essential element for living cells. The present study tested the ability of different concentrations of Si G57™ to induce neuroprotection or neurotoxicity over 24 h in the SH-SY5Y human neuroblastoma cell line. Cell viability, cellular proliferation, LDH release, ROS, antioxidant capacity, TBARS, caspase-3, -8 and -9, DNA fragmentation, and TNF-α levels were evaluated. Low Si doses (50-250 ng mL(-1)) increased the cell viability and reduced caspase-3 and -8 activities and TNF-α level. The increase in cell viability was independent of any proliferative effect as there was no variation in cyclin E and PCNA levels. At higher concentrations, Si increased caspase-3, as well as TBARS, LDH, DNA fragmentation, and TNF-α releases. Altogether, these results suggest that Si could act either as a neuroprotector or a neurotoxic agent depending on the concentration tested. This study emphasizes the importance of developing new neuroprotective therapies based on low Si doses.
PB Elsevier
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/116411
UL https://hdl.handle.net/20.500.14352/116411
LA eng
NO Garcimartín A, Merino JJ, Santos-López JA, López-Oliva ME, González MP, Sánchez-Muniz FJ, Benedí J. Silicon as neuroprotector or neurotoxic in the human neuroblastoma SH-SY5Y cell line. Chemosphere. 2015 Sep;135:217-24. doi: 10.1016/j.chemosphere.2015.04.060. Epub 2015 May 15. PMID: 25957141.
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 6 jul 2025