RT Journal Article
T1 Alcohol binge drinking induces downregulation of blood-brain barrier proteins in the rat frontal cortex -but not in the hippocampus- that is not prevented by OEA pretreatment
A1 Rodríguez-González, Alicia
A1 Moya Montes, Marta
A1 Rodríguez De Fonseca, Fernando Antonio
A1 Gómez De Heras, María Raquel
A1 Orio Ortiz, Laura
AB Alcohol binge drinking promotes neuroinflammation which could be partially mediated by the passage of ABD-induced peripheral inflammatory molecules to the brain parenchyma through the blood-brain barrier. The BBB is sealed by tight junction proteins, which regulate the access of substances to the brain. Whether ABD alters the BBB or not remains controversial. Here, we measured the expression of BBB proteins in frontal cortex and hippocampus after an ABDprocedure that was previously shown to induce neuroinflammation in the FC, and checked neuroinflammation in the hippocampus. Oleoylethanolamide is known to inhibit ABD-induced neuroinflammation in rat FC but the mechanisms ofaction are not clear: whereas OEA protects against alcohol-induced breakdown of the TJ proteins in the gut barrier reducing peripheral inflammation, its effect in the TJ of the BBB remains unknown. Here, we studied whether OEA (5mg/kg, before each gavage) prevented alcohol-induced BBB dysfunction by measuring the expression of zona-occludens, occludin, and laminin in FCand hippocampus. ABD animals showed reduced laminin and occludin levels in the FC, indicative of BBB dysfunction, which is concordant with previous findings showing ABDinduced neuroinflammation in this brain region. OEA did not prevent ABDinduced changes in the BBB proteins in the FC, suggesting that the OEA main mechanism of action to inhibit neuroinflammation in this brain region is not related to prevention of TJ proteins alteration in the BBB. In the hippocampus, this ABD protocol did not alter BBB protein levels and no markers ofneuroinflammation were found elevated.
PB Frontiers Publishing Partnerships
YR 2023
FD 2023-02-24
LK https://hdl.handle.net/20.500.14352/101997
UL https://hdl.handle.net/20.500.14352/101997
LA eng
NO Rodríguez-González A, Moya M, Rodríguez de Fonseca F, Gómez de Heras R, Orio L* (2023). Alcohol binge drinking induces downregulation of blood-brain barrier proteins in the rat frontal cortex -but not in the hippocampus- that is not prevented by OEA pretreatment. Adv. Drug. Alco. Res. 3:11091.
NO FEDER (European Union)/Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación (Spain), grant numbers: RTI2018-099535-BI00 and PID-2021-127256OB-100 to LO
NO Instituto de Salud Carlos III (ISCIII) and the Next-Generation EU fonds, which finances the MRR (“mecanismo para la recuperación y la resiliencia”) actions, grant number: RD21/0009/0027
NO AR-G was a recipient of a fellowship from Consejería de Educación, Juventud y Deporte (Comunidad de Madrid/Fondo Social Europeo).
DS Docta Complutense
RD 21 abr 2025