RT Journal Article
T1 Neospora caninum infection during early pregnancy in cattle: how the isolate influences infection dynamics, clinical outcome and peripheral and local immune responses
A1 Regidor-Cerrillo, Javier
A1 Arranz Solís, David
A1 Benavides, Julio
A1 Gómez Bautista, Mercedes
A1 Castro-Hermida, José Antonio
A1 Mezo, Mercedes
A1 Pérez, Valentín
A1 Ortega Mora, Luis Miguel
A1 González-Warleta, Marta
AB This work studies the influence of Neospora caninum intra-species diversity on abortion outcome, infection dynamics in terms of parasite dissemination and peripheral-local immune responses in pregnant cattle. Animals were intravenously inoculated at day 70 of pregnancy with 107 tachyzoites of two isolates showing marked differences in virulence in vitro and in pregnant mouse models: Nc-Spain7, a high virulence isolate, and Nc-Spain8, a low-to-moderate virulence isolate. After inoculation, pregnancy was monitored, and dams were culled when foetal death was detected. Foetal mortality occurred in all infected heifers between days 24 and 49 post-infection (pi), however, it was detected sooner in Nc-Spain7-infected animals (median day = 34) than those inoculated with Nc-Spain8 (median day = 41) with a trend towards significance (P < 0.11). Similar histological lesions were observed in placentomes and in most of the foetuses from the two infected groups. However, parasites were more frequently detected in the placenta and foetuses by PCR and in the foetal brain by immunohistochemistry in Nc-Spain7-infected animals. Specific antibodies were detected starting at day 13 post-infection in all infected cattle, with higher IgG levels in Nc-Spain7-infected group. IFN-γ and IL-4 profiles also varied between infected groups in PBMC stimulation assays. Infected animals showed significant increases in their cytokine mRNA levels (IFN-γ, IL-4, IL-10, IL-12p40 and TNF-α) in the caruncle at time of foetal death. Differences between the infected groups were also observed for cytokine profiles. These results demonstrate the influence of the N. caninum isolate on foetal death outcome, infection dynamics and immune responses in cattle.
PB BMC
SN 1297-9716
YR 2014
FD 2014-01-30
LK https://hdl.handle.net/20.500.14352/91489
UL https://hdl.handle.net/20.500.14352/91489
LA eng
NO Regidor-Cerrillo, J., Arranz-Solís, D., Benavides, J. et al. Neospora caninum infection during early pregnancy in cattle: how the isolate influences infection dynamics, clinical outcome and peripheral and local immune responses. Vet Res 45, 10 (2014). https://doi.org/10.1186/1297-9716-45-10
NO FECYT -- INFLUENCIA DE LA VARIABILIDAD INTRAESPECIE DE Neospora caninum ...
NO JAE-Doc program, financed in part by the European Social Fund (ESF)
NO INIA
DS Docta Complutense
RD 12 abr 2025