RT Journal Article T1 Green Extraction Technologies and Kombucha Elaboration Using Strawberry Tree (Arbutus unedo) Fruits to Obtain Antioxidant and Anti-Inflammatory Fractions A1 Siles-Sánchez, María de las Nieves A1 Tejedor Calvo, Eva A1 Jaime, Laura A1 Santoyo, Susana A1 Morales Hernández, Diego AB The fruits of Arbutus unedo (strawberry tree, ST) have been widely used for culinary and medicinal purposes because of their content in phenolic compounds and other bioactives. The aim of this study was to apply green technologies using water as solvent to obtain phenolic-rich fractions from ST fruits. Moreover, a kombucha beverage (KOM) using these fruits as substrate was prepared. Conventional (cold and hot water extractions) and advanced (ultrasound- and microwave-assisted-extractions) protocols were carried out, leading to fractions with high total phenolic content (TPC), up to 40.72 mg/100 mL in a microwave extract (MHWE), with also 7.89 mg total carbohydrates/mL and 106.12 µg soluble proteins/mL. Moreover, the kombucha showed even higher TPC (70.99 mg/100 mL). Biological activities were also tested for all the fractions, registering the highest antioxidant activity for KOM (2.63 and 6.22 µmol/mL of TEAC value as DPPH• scavenger and cellular antioxidant activity, respectively) and MHWE (2.76 µmol/mL of TEAC value). Strong correlations were observed between TPC or specific compounds (mainly catechin derivatives) and antioxidant activity. Furthermore, KOM and the aqueous extracts were able to reduce pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) in a cellular model. PB Springer SN 1935-5130 YR 2024 FD 2024-06-07 LK https://hdl.handle.net/20.500.14352/105681 UL https://hdl.handle.net/20.500.14352/105681 LA eng NO Siles-Sánchez, M., Tejedor-Calvo, E., Jaime, L. et al. Green Extraction Technologies and Kombucha Elaboration Using Strawberry Tree (Arbutus unedo) Fruits to Obtain Antioxidant and Anti-Inflammatory Fractions. Food Bioprocess Technol (2024). https://doi.org/10.1007/s11947-024-03451-8 NO Ministerio de Ciencia e Innovación (España) DS Docta Complutense RD 13 abr 2025